Name: flash freezing and cryosectioning e12.5 mouse brain
Uploaded: 2007-05-28T00:00:00
Description: Watch this Scientific Journal Video about Flash Freezing and Cryosectioning E12.5 Mouse Brain at JoVE.com

<ol><li> Fissare il tessuto in paraformaldeide 4% in PBS per il tempo desiderato. </li><li> Saccarosio infondere tessuto (crioprotezione) <ol><li> Preparare la soluzione di saccarosio al 30% in PBS w / v nel 2059 tubo. </li><li> Lavare il tessuto in PBS 3x (~ 5 min con dondolo). </li><li> Tessuto posto in soluzione di saccarosio al 30%. Tessuto non affonderà. </li><li> Posizionare il tessuto in 4 ° C durante la notte, o fino a quando è affondata. </li></ol></li><li> Dimensione etichetta appropriata cryomold con informazioni e orientamento. <...

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		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
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<td>Tissue-Tek Cryomold</td>
<td>&nbsp;</td>
<td>Ted Pella, Inc.</td>
<td>27181</td>
<td>&nbsp;</td>
<tr>
<td>O.C.T.</td>
<td>&nbsp;</td>
<td>Ted Pella, Inc</td>
<td>27050</td>
<td>&nbsp;</td>
<tr>
<td>Sucrose solution</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>30% sucrose solution in PBS w/v</td>
</tr>
<tr>
<td>paraformaldehyde</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>4% paraformaldehyde in PBS </td>
</tr>		</tbody>
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flash freezing and cryosectioning e12.5 mouse brain

Watch this Scientific Journal Video about Flash Freezing and Cryosectioning E12.5 Mouse Brain at JoVE.com

Flash Freezing and Cryosectioning E12.5 Mouse Brain

<p class="jove_content">Dimostrato in questo video sono le tecniche per flash congelamento e sezionamento del tessuto embrionale del cervello di topo. Consigli utili per l&#39;utilizzo del criostato sono dati, compresi i metodi di risoluzione dei problemi che possono essere utilizzati durante il taglio per assicurare che le sezioni risultanti tessuti sono privi di crepe e altre distorsioni.</p>

Flash Congelamento e criosezionamento E12.5 cervello di topo

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