Name: flash freezing and cryosectioning e12.5 mouse brain
Uploaded: 2007-05-28T00:00:00
Description: Watch this Scientific Journal Video about Flash Freezing and Cryosectioning E12.5 Mouse Brain at JoVE.com

<ol><li> 원하는 시간을 PBS에 4 % paraformaldehyde에 조직을 수정. </li><li> 자당 달이다 조직 (cryoprotection) <ol><li> 2,059 튜브 PBS W / V에서 30 % 자당 솔루션을 확인하십시오. </li><li> PBS에서 조직 배 린스 (락을 함께 ~ 5 분). </li><li> 30 % 자당 용액에 넣어 조직. 조직은 침몰하지 않습니다. </li><li> 그것이 침몰했다 ° C 야간, 또는 때까지 4 조직을 놓으십시오. </li></ol></li><li> 라벨 적절한 크기는 정보와 오리 엔테이션과 함께 cryomold. </...

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en">	
		
		<div>
		<table border="1">
		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>Tissue-Tek Cryomold</td>
<td>&nbsp;</td>
<td>Ted Pella, Inc.</td>
<td>27181</td>
<td>&nbsp;</td>
<tr>
<td>O.C.T.</td>
<td>&nbsp;</td>
<td>Ted Pella, Inc</td>
<td>27050</td>
<td>&nbsp;</td>
<tr>
<td>Sucrose solution</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>30% sucrose solution in PBS w/v</td>
</tr>
<tr>
<td>paraformaldehyde</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>4% paraformaldehyde in PBS </td>
</tr>		</tbody>
		</table>
		</div>
			<div>
					</div>
		

flash freezing and cryosectioning e12.5 mouse brain

Watch this Scientific Journal Video about Flash Freezing and Cryosectioning E12.5 Mouse Brain at JoVE.com

Flash Freezing and Cryosectioning E12.5 Mouse Brain

<p class="jove_content">플래시 마우스 배아 뇌 조직을 동결 sectioning위한 방법은 다음과 같습니다이 비디오에서 보여주었다. 그라 이오 스탯를 사용하기위한 유용한 팁을은 결과 조직의 섹션 균열 및 기타 왜곡 무료로되는 것을 보장하기 위해 절단하는 동안 사용할 수있는 문제 해결 방법을 포함하여 제공됩니다.</p>

플래시 동결 E12.5 마우스 브레인 Cryosectioning

Research

JoVE Journal

Biology

Freezing Human ES Cells

냉동 인간 ES 세포

Here we demonstrate how our lab freezes HuES human embryonic stem cell lines.  A healthy, exponentially expanding culture is washed with PBS to remove ...

연구

생물학

Freezing, Thawing, and Packaging Cells for Transport

, 동결 해동 및 운송을위한 포장 셀

Cultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the ...

Freezing and Thawing Human Embryonic Stem Cells

인간 배아 줄기 세포를 동결 융해

Since James Thomson et al developed a technique in 1998 to isolate and grow hES in culture, freezing cells for later use and thawing and expanding cells ...

<em>In vivo</em> Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

<em>생체내</em> 이미징 및 난소암의 Orthotopic 마우스 모델에서 치료 트리 <em>트먼트</em>

Human cancer and response to therapy is better represented in orthotopic animal models.  This paper describes the development of an orthotopic mouse model ...

ReAsH/FlAsH Labeling and Image Analysis of Tetracysteine Sensor Proteins in Cells

ReAsH / 플래시 라벨링 및 전지 Tetracysteine​​ 센서 단백질의 이미지 분석

Fluorescent proteins and dyes are essential tools for the study of protein trafficking, localization and function in cells.  While fluorescent 
proteins ...

Cryosectioning Yeast Communities for Examining Fluorescence Patterns

형광 패턴 검사를위한 효모 커뮤니티 Cryosectioning

Microbes typically live  in communities. The spatial organization  of cells within a community is believed to impact the survival and function of the  ...

Tissue Triage and Freezing for Models of Skeletal Muscle Disease

조직의 우선 순위 구분과 골격근 질환 모델에 대한 동결

Skeletal muscle is a unique tissue because of its structure and function, which requires specific protocols for tissue collection to obtain optimal ...

Cryosectioning Method for Microdissection of Murine Colonic Mucosa

쥐 대장 점막의 이외에 Microdissection을위한 냉동 절편 방법

The colonic mucosal tissue provides a vital barrier to luminal antigens. This barrier is composed of a monolayer of simple columnar epithelial cells. The ...

Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

유전자 발현 및 조직 학적 분석을위한 단일 마우스 골격근의 연속 지역의 냉동 절편

With this method, consecutive cryosections are collected to enable both microscopy applications for tissue histology and enrichment of RNA for gene ...

Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy

플래시 및 동결 : 전자 현미경으로 막 역학을 캡처하는 소설 기법

Cells constantly change their membrane architecture and protein distribution, but it is extremely difficult to visualize these events at a temporal and ...