Name: flash freezing and cryosectioning e12.5 mouse brain
Uploaded: 2007-05-28T00:00:00
Description: Watch this Scientific Journal Video about Flash Freezing and Cryosectioning E12.5 Mouse Brain at JoVE.com

<ol><li> Fix tecido em paraformaldeído 4% em PBS para o tempo desejado. </li><li> Tecido sacarose infundir (crioproteção) <ol><li> Fazer solução de sacarose 30% em PBS w / v em 2059 tubo. </li><li> Enxaguar tecido em 3x PBS (~ 5 min com balanço). </li><li> Tecido lugar em solução de sacarose 30%. Tecido não vai afundar. </li><li> Coloque o tecido em 4 ° C durante a noite, ou até que tenha afundado. </li></ol></li><li> Tamanho da etiqueta apropriada cryomold com informação e orientação. </li><li> Preencha cryomold com outubro (evi...

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		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
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<tr>
<td>Tissue-Tek Cryomold</td>
<td>&nbsp;</td>
<td>Ted Pella, Inc.</td>
<td>27181</td>
<td>&nbsp;</td>
<tr>
<td>O.C.T.</td>
<td>&nbsp;</td>
<td>Ted Pella, Inc</td>
<td>27050</td>
<td>&nbsp;</td>
<tr>
<td>Sucrose solution</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>30% sucrose solution in PBS w/v</td>
</tr>
<tr>
<td>paraformaldehyde</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>4% paraformaldehyde in PBS </td>
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flash freezing and cryosectioning e12.5 mouse brain

Watch this Scientific Journal Video about Flash Freezing and Cryosectioning E12.5 Mouse Brain at JoVE.com

Flash Freezing and Cryosectioning E12.5 Mouse Brain

<p class="jove_content">Demonstrado neste vídeo são as técnicas de congelamento instantâneo e secção tecido cerebral embrionário de rato. Dicas úteis para usar o criostato são dadas, incluindo métodos de solução de problemas que podem ser usados ​​durante o corte para garantir que as seções resultantes tecidos estão livres de rachaduras e outras distorções.</p>

Flash Freezing e Cryosectioning E12.5 cérebro de camundongo

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