Name: flash freezing and cryosectioning e12.5 mouse brain
Uploaded: 2007-05-28T00:00:00
Description: Watch this Scientific Journal Video about Flash Freezing and Cryosectioning E12.5 Mouse Brain at JoVE.com

<ol><li> Fix ткани в 4% параформальдегида в ФСБ на необходимое время. </li><li> Сахароза влить ткани (криозащиты) <ol><li> Сделать 30% раствор сахарозы в ФБР м / о в 2059 трубке. </li><li> Промойте ткань в 3 раза PBS (~ 5 мин с качалки). </li><li> Место ткани в 30% раствором сахарозы. Ткань не утонет. </li><li> Место ткани в 4 ° С в течение ночи...

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		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
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<tr>
<td>Tissue-Tek Cryomold</td>
<td>&nbsp;</td>
<td>Ted Pella, Inc.</td>
<td>27181</td>
<td>&nbsp;</td>
<tr>
<td>O.C.T.</td>
<td>&nbsp;</td>
<td>Ted Pella, Inc</td>
<td>27050</td>
<td>&nbsp;</td>
<tr>
<td>Sucrose solution</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>30% sucrose solution in PBS w/v</td>
</tr>
<tr>
<td>paraformaldehyde</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>4% paraformaldehyde in PBS </td>
</tr>		</tbody>
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flash freezing and cryosectioning e12.5 mouse brain

Watch this Scientific Journal Video about Flash Freezing and Cryosectioning E12.5 Mouse Brain at JoVE.com

Flash Freezing and Cryosectioning E12.5 Mouse Brain

<p class="jove_content">Продемонстрированный в этом видео-методы для флеш-замораживания и секционирования эмбриональной ткани мозга от мыши. Полезные советы по использованию криостат даны, в том числе способы устранения неполадок, которые могут использоваться при резке, чтобы результирующая разделах ткани без трещин и других искажений.</p>

Флэш Замораживание и Cryosectioning E12.5 мозга мыши

Research

JoVE Journal

Biology

Freezing Human ES Cells

Замораживание клеток человека ES

Here we demonstrate how our lab freezes HuES human embryonic stem cell lines.  A healthy, exponentially expanding culture is washed with PBS to remove ...

Freezing, Thawing, and Packaging Cells for Transport

Замораживание, размораживание, и упаковка Клетки для транспорта

Cultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the ...

Freezing and Thawing Human Embryonic Stem Cells

Замораживание и размораживание человеческих эмбриональных стволовых клеток

Since James Thomson et al developed a technique in 1998 to isolate and grow hES in culture, freezing cells for later use and thawing and expanding cells ...

<em>In vivo</em> Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

<em>В естественных</em> изображений и лечебных процедур в Ортотопическая мышиной модели рака яичников

Human cancer and response to therapy is better represented in orthotopic animal models.  This paper describes the development of an orthotopic mouse model ...

ReAsH/FlAsH Labeling and Image Analysis of Tetracysteine Sensor Proteins in Cells

ReAsH / Flash Маркировка и анализ изображений белков Tetracysteine ​​датчика в клетках

Fluorescent proteins and dyes are essential tools for the study of protein trafficking, localization and function in cells.  While fluorescent 
proteins ...

Cryosectioning Yeast Communities for Examining Fluorescence Patterns

Cryosectioning дрожжи Сообщества для экспертизы флуоресценции Patterns

Microbes typically live  in communities. The spatial organization  of cells within a community is believed to impact the survival and function of the  ...

Tissue Triage and Freezing for Models of Skeletal Muscle Disease

Ткань Сортировка и морозильные моделей скелетных мышечных заболеваний

Skeletal muscle is a unique tissue because of its structure and function, which requires specific protocols for tissue collection to obtain optimal ...

Cryosectioning Method for Microdissection of Murine Colonic Mucosa

Cryosectioning Метод микродиссекции мышиной слизистой оболочки толстой кишки

The colonic mucosal tissue provides a vital barrier to luminal antigens. This barrier is composed of a monolayer of simple columnar epithelial cells. The ...

Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

Cryosectioning соприкасающихся регионов скелетной мышцы одной мыши для экспрессии генов и гистологических анализов

With this method, consecutive cryosections are collected to enable both microscopy applications for tissue histology and enrichment of RNA for gene ...

Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy

Флэш-и-Фриз: Роман Техника для захвата мембраны динамика с электронной микроскопии

Cells constantly change their membrane architecture and protein distribution, but it is extremely difficult to visualize these events at a temporal and ...