Name: flash freezing and cryosectioning e12.5 mouse brain
Uploaded: 2007-05-28T00:00:00
Description: Watch this Scientific Journal Video about Flash Freezing and Cryosectioning E12.5 Mouse Brain at JoVE.com

<ol><li> Fijar el tejido en el 4% paraformaldehido en PBS durante el tiempo deseado. </li><li> Sacarosa infundir tejido (crioprotector) <ol><li> Hacer 30% de solución de sacarosa en PBS w / v en 2059 tubo. </li><li> Enjuague el tejido 3 veces en PBS (~ 5 min con la oscilación). </li><li> Coloque el tejido en una solución de sacarosa al 30%. Tejido no se hunda. </li><li> Coloque el tejido en 4 ° C durante la noche, o hasta que se ha hundido. </li></ol></li><li> Tamaño de la etiqueta apropiada cryomold con información y orientación. </li><...

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		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
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<tr>
<td>Tissue-Tek Cryomold</td>
<td>&nbsp;</td>
<td>Ted Pella, Inc.</td>
<td>27181</td>
<td>&nbsp;</td>
<tr>
<td>O.C.T.</td>
<td>&nbsp;</td>
<td>Ted Pella, Inc</td>
<td>27050</td>
<td>&nbsp;</td>
<tr>
<td>Sucrose solution</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>30% sucrose solution in PBS w/v</td>
</tr>
<tr>
<td>paraformaldehyde</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>4% paraformaldehyde in PBS </td>
</tr>		</tbody>
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flash freezing and cryosectioning e12.5 mouse brain

Watch this Scientific Journal Video about Flash Freezing and Cryosectioning E12.5 Mouse Brain at JoVE.com

Flash Freezing and Cryosectioning E12.5 Mouse Brain

<p class="jove_content">Se demuestra en este vídeo son las técnicas de congelación para el flash y el corte del tejido cerebral de embriones de ratón. Consejos útiles para el uso del criostato se les da, incluyendo los métodos de solución de problemas que se pueden utilizar durante el corte para asegurar que las secciones de tejidos resultantes están libres de grietas y otras distorsiones.</p>

Flash de congelación y muestras criostáticas E12.5 cerebro de ratón

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Skeletal muscle is a unique tissue because of its structure and function, which requires specific protocols for tissue collection to obtain optimal ...

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With this method, consecutive cryosections are collected to enable both microscopy applications for tissue histology and enrichment of RNA for gene ...

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