Name: flash freezing and cryosectioning e12.5 mouse brain
Uploaded: 2007-05-28T00:00:00
Description: Watch this Scientific Journal Video about Flash Freezing and Cryosectioning E12.5 Mouse Brain at JoVE.com

<ol><li> Fix vävnad i 4% paraformaldehyd i PBS för önskad tid. </li><li> Sackaros ingjuta vävnad (cryoprotection) <ol><li> Gör 30% sackaroslösning i PBS w / v under 2059 rör. </li><li> Skölj vävnad 3x i PBS (~ 5 min med gungande). </li><li> Placera vävnad i 30% sackaroslösning. Tissue kommer inte att sjunka. </li><li> Placera vävnad i 4 ° C över natten, eller tills den har sjunkit. </li></ol></li><li> Etikett lämplig storlek cryomold med information och orientering. </li><li> Fyll cryomold med oktober (undvik bubblor). </li><li> ...

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en">	
		
		<div>
		<table border="1">
		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>Tissue-Tek Cryomold</td>
<td>&nbsp;</td>
<td>Ted Pella, Inc.</td>
<td>27181</td>
<td>&nbsp;</td>
<tr>
<td>O.C.T.</td>
<td>&nbsp;</td>
<td>Ted Pella, Inc</td>
<td>27050</td>
<td>&nbsp;</td>
<tr>
<td>Sucrose solution</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>30% sucrose solution in PBS w/v</td>
</tr>
<tr>
<td>paraformaldehyde</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>4% paraformaldehyde in PBS </td>
</tr>		</tbody>
		</table>
		</div>
			<div>
					</div>
		

flash freezing and cryosectioning e12.5 mouse brain

Watch this Scientific Journal Video about Flash Freezing and Cryosectioning E12.5 Mouse Brain at JoVE.com

Flash Freezing and Cryosectioning E12.5 Mouse Brain

<p class="jove_content">Visats i denna video är de tekniker för flash frysning och sektionering embryonal hjärnvävnad från mus. Användbara tips för att använda kryostat ges, inklusive felsökning metoder som kan användas samtidigt minska för att säkerställa att den resulterande vävnaderna sektionerna är fria från sprickor och andra störningar.</p>

Flash Frysning och Cryosectioning E12.5 Mouse Brain

Research

JoVE Journal

Biology

Freezing Human ES Cells

Frysning Mänskliga ES-celler

Here we demonstrate how our lab freezes HuES human embryonic stem cell lines.  A healthy, exponentially expanding culture is washed with PBS to remove ...

Freezing, Thawing, and Packaging Cells for Transport

Frysning, upptining och celler Förpackning för transport

Cultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the ...

Freezing and Thawing Human Embryonic Stem Cells

Nedfrysning och upptining mänskliga embryonala stamcellslinjer

Since James Thomson et al developed a technique in 1998 to isolate and grow hES in culture, freezing cells for later use and thawing and expanding cells ...

<em>In vivo</em> Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

<em>In vivo</em> Imaging och terapeutiska behandlingar i en Orthotopic musmodell av äggstockscancer

Human cancer and response to therapy is better represented in orthotopic animal models.  This paper describes the development of an orthotopic mouse model ...

ReAsH/FlAsH Labeling and Image Analysis of Tetracysteine Sensor Proteins in Cells

ReAsH / Flash märkning och bildanalys av proteiner Tetracysteine ​​sensor i cellerna

Fluorescent proteins and dyes are essential tools for the study of protein trafficking, localization and function in cells.  While fluorescent 
proteins ...

Cryosectioning Yeast Communities for Examining Fluorescence Patterns

Cryosectioning Jäst gemenskapernas för att pröva Fluorescence mönster

Microbes typically live  in communities. The spatial organization  of cells within a community is believed to impact the survival and function of the  ...

Tissue Triage and Freezing for Models of Skeletal Muscle Disease

Vävnads Triage och frysning efter Modeller av skelettmuskulatur Sjukdom

Skeletal muscle is a unique tissue because of its structure and function, which requires specific protocols for tissue collection to obtain optimal ...

Cryosectioning Method for Microdissection of Murine Colonic Mucosa

Cryosectioning Förfarande för Microdissection av murin kolonslemhinna

The colonic mucosal tissue provides a vital barrier to luminal antigens. This barrier is composed of a monolayer of simple columnar epithelial cells. The ...

Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

Cryosectioning angränsande regionerna i samma mus skelettmuskulatur för genuttryck och histologiska analyser

With this method, consecutive cryosections are collected to enable both microscopy applications for tissue histology and enrichment of RNA for gene ...

Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy

Flash-and-Freeze: En ny teknik för att fånga Membrane Dynamics med elektronmikroskopi

Cells constantly change their membrane architecture and protein distribution, but it is extremely difficult to visualize these events at a temporal and ...