Name: flash freezing and cryosectioning e12.5 mouse brain
Uploaded: 2007-05-28T00:00:00
Description: Watch this Scientific Journal Video about Flash Freezing and Cryosectioning E12.5 Mouse Brain at JoVE.com

<ol><li> Istenilen zaman için PBS içinde% 4 paraformaldehid doku sabitleyin. </li><li> Sakkaroz demlenmeye doku (cryoprotection) <ol><li> 2059 tüp PBS w / v% 30 sakaroz çözeltisini olun. </li><li> PBS içinde doku 3x sallanan ile durulayın (~ 5 dk). </li><li> % 30 sukroz solüsyonu yerleştirin doku. Doku lavabo olmaz. </li><li> Batırdı, 4 ° C gecede kadar veya doku yerleştirin. </li></ol></li><li> Etiket uygun boyutu, bilgi ve yönlendirme ile cryomold. </li><li> (Baloncuklar önlemek) OCT ile cryomold doldurun. </li><li> Ekim banyo ...

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		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
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<td>Tissue-Tek Cryomold</td>
<td>&nbsp;</td>
<td>Ted Pella, Inc.</td>
<td>27181</td>
<td>&nbsp;</td>
<tr>
<td>O.C.T.</td>
<td>&nbsp;</td>
<td>Ted Pella, Inc</td>
<td>27050</td>
<td>&nbsp;</td>
<tr>
<td>Sucrose solution</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>30% sucrose solution in PBS w/v</td>
</tr>
<tr>
<td>paraformaldehyde</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>4% paraformaldehyde in PBS </td>
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flash freezing and cryosectioning e12.5 mouse brain

Watch this Scientific Journal Video about Flash Freezing and Cryosectioning E12.5 Mouse Brain at JoVE.com

Flash Freezing and Cryosectioning E12.5 Mouse Brain

<p class="jove_content">Bu video flaş fare embriyonik beyin dokusu dondurma ve kesit alma teknikleri vardır gösterdi. Ortaya çıkan doku bölümleri çatlak ve diğer çarpıtmalar olduğundan emin olmak için kesim sırasında kullanılabilir sorun giderme yöntemleri de dahil olmak üzere, Kriyostat kullanmak için yararlı ipuçları verilmiştir.</p>

Flaş Dondurma ve E12.5 Fare Beyin Cryosectioning

Research

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