Name: in vivo imaging of transgenic leishmania parasites in a live host
Uploaded: 2010-07-27T17:00:00
Description: Watch this Scientific Journal Video about In vivo Imaging of Transgenic Leishmania Parasites in a Live Host at JoVE.com

这项工作是从退伍军人事务部的优异审查授予部分经费，由美国国立卫生研究院拨款AI045540，AI067874，AI076233 - 01和AI080801（水电部），和AI29646（SMB）。这项工作是在CT和JG的经费由美国国立卫生研究院的T32 AI07511部分。

1。小动物转基因利什曼原虫感染 1。寄生虫线转基因利什曼原虫 SPP。寄生虫表达荧光素酶的生成使用episomal或作为一个整合载体。1 2克隆线是首选。两个要点是： （一）综合超过episomal荧光素酶的荧光素酶是首选，因为这些寄生虫线，在理论上应该更好地保留在药物的压力，即没有引入到哺乳动物的基因。然而，?...

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在活体成像系统（IVIS），为整个动物的成像或体内成像实验感染利什曼病的不同形式的模型的 方法。18,16利什曼原虫SPP。寄生虫，可设计，以表达萤火虫荧光素酶在体内 IVIS成像技术检测的水平。这种方法的主要优势之一是它允许利什曼原虫属非侵入性可视化。活的动物宿主内。该方法已应用于其他传染性疾病模型，传染性病原体可以表达的转基因?...

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		<th>Material Name</th>
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		<th>Comment</th>
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<td>D-Luciferin Potassium Salt </td>
<td>Reagent</td>
<td>Caliper LifeSciences (Formerly Xenogen)</td>
<td>122796</td>
<td>&nbsp;</td>
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<td>IVIS Imaging System 200 Series</td>
<td>Equipment</td>
<td>Caliper LifeSciences</td>
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<td>Other IVIS models that can be used include: Lumina II, Lumina XR, Kinetic, and Spectrum. </td>
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in vivo imaging of transgenic leishmania parasites in a live host

独特品种<em&gt;利什曼原虫，</em&gt;一个家庭的原生动物寄生虫<em&gt; Trypanosomatidae</em&gt;，通常会导致不同的人类疾病的表现。疾病最常见的形式是内脏利什曼病（VL）和皮肤利什曼病（CL）。利什曼病的小鼠模型被广泛使用，但在小鼠疾病的寄生虫负担的量化要求的小鼠在感染后的不同时间安乐死。寄生负载，然后在显微镜下测量，限制稀释实验中，或寄生虫的DNA定量PCR扩增。 “<em&gt;在体内</em&gt;成像系统（IVIS）有一个集成的软件包，使细胞在生物体相关的发光信号的检测。既可以减少动物的使用和纵向遵循感染在体内成像模型在个人，<em&gt;利什曼原虫</em&gt; SPP。造成VL或CL成立。寄生虫设计，以表达荧光素酶，这些被引入到小鼠皮下或静脉注射。转基因荧光素酶驾驶的生物发光的定量测量<em&gt;利什曼原虫</em使用IVIS&gt;鼠标内的寄生虫。个人小鼠可成像多次在纵向研究中，让我们评估在最初的实验寄生虫接种的动物间的变化，并评估在小鼠组织中的寄生虫的繁殖。寄生虫检测灵敏度高，与皮肤的位置。虽然这是极有可能的信号（光子/秒/寄生虫）是在更深的内脏器官比皮肤低，但信号的定量比较肤浅与深刻的网站还没有完成。这是可能的部位的寄生虫之间的数字不能直接比较，但可以在同一组织中的寄生虫负载之间的小鼠相比。一个visceralizing物种的例子（<em&gt; L。 infantum chagasi</em&gt;）和一个物种造成皮肤利什曼病（<em&gt; L。墨西哥</em&gt;）所示。 IVIS程序可用于监测和分析各种各样的小动物模型<em&gt;利什曼原虫</em&gt;物种造成人类利什曼病的不同形式。

Watch this Scientific Journal Video about In vivo Imaging of Transgenic Leishmania Parasites in a Live Host at JoVE.com

In vivo Imaging of Transgenic Leishmania Parasites in a Live Host

一个<em&gt;在体内</em&gt;成像系统是用来生成的定量测量与Trypanosomatid原虫感染小鼠<em&gt;利什曼原虫</em&gt;。这是一个慢性的过程中整个检测在许多组织中表达的荧光素酶的寄生虫的非侵入性和非致命性的方法<em&gt;利什曼原虫</em&gt; SPP。感染。

在Live主机转利什曼原虫寄生虫在体内成像

Distinct species of Leishmania, a protozoan parasite of the family Trypanosomatidae, typically cause different human disease manifestations. The most ...

Research

JoVE Journal

Immunology and Infection

In vivo Imaging of Transgenic Leishmania Parasites in a Live Host

Non-invasive Imaging of Leukocyte Homing and Migration in vivo

Non-invasive Imaging of Leukocyte Homing and Migration in vivo

白细胞的归位和迁移的非侵入性的成像在体内</em

Two-photon (2P) microscopy is a high resolution imaging technique that has been broadly adapted by biologists.  The value of 2P microscopy is that it ...

科研

免疫与感染

Use of an Optical Trap for Study of Host-Pathogen Interactions for Dynamic Live Cell Imaging

动态活细胞成像的宿主 - 病原体相互作用的研究使用的光学陷阱

Dynamic live cell imaging allows direct visualization of real-time interactions between cells of the immune system1, 2; however, the lack of spatial and ...

Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model

Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model

移植物抗宿主病的诱导和<em在体内</em&gt; T细胞监测中的应用MHC匹配的小鼠模型

Graft-versus-host disease (GVHD) is the limiting barrier to the broad use of bone marrow transplant as a curative therapy for a variety of hematological ...

Human Placental and Decidual Organ Cultures to Study Infections at the Maternal-fetal Interface

人胎盘及蜕膜器官培养研究在母胎界面感染

The placenta shows a large degree of interspecies anatomic variability. To best understand biology and pathophysiology of the human placenta, it is ...

Quantification of Intracellular Growth Inside Macrophages is a Fast and Reliable Method for Assessing the Virulence of Leishmania Parasites

Quantification of Intracellular Growth Inside Macrophages is a Fast and Reliable Method for Assessing the Virulence of Leishmania Parasites

巨噬细胞内细胞内生长的定量化是评价利什曼原虫寄生虫毒力的快速可靠方法

The lifecycle of Leishmania, the causative agent of leishmaniasis, alternates between promastigote and amastigote stages inside the insect and vertebrate ...

In Vivo Imaging of Reactive Oxygen Species in a Murine Wound Model

In Vivo Imaging of Reactive Oxygen Species in a Murine Wound Model

在 vivo小鼠创面模型中活性氧的成像

The generation of reactive oxygen species (ROS) is a hallmark of inflammatory processes, but in excess, oxidative stress is widely implicated in various ...

Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium

人类舱内单聚物研究病原体、共脉和宿主肠道上皮细胞之间的相互作用

Human 3-dimensional (3D) enteroid or colonoid cultures derived from crypt base stem cells are currently the most advanced ex vivo model of the intestinal ...

In Vivo Infection with Leishmania amazonensis to Evaluate Parasite Virulence in Mice

In Vivo Infection with Leishmania amazonensis to Evaluate Parasite Virulence in Mice

在 Vivo 感染利什曼病亚马孙病评估小鼠的寄生虫毒性

Leishmania spp. are protozoan parasites that cause leishmaniases, diseases that present a wide spectrum of clinical manifestations from cutaneous to ...

Live Imaging of Chemokine Receptors in Zebrafish Neutrophils During Wound Responses

在伤口反应期间斑马鱼中性粒细胞中趋化因子受体的实时成像

Leukocyte guidance by chemical gradients is essential for immune responses. Neutrophils are the first cells to be recruited to sites of tissue damage ...

Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice

Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice

由加德纳菌阴道膀胱暴露在小鼠中引发的复发性大肠杆菌尿路感染

Recurrent urinary tract infections (rUTI) caused by uropathogenic Escherichia coli (UPEC) are common and costly. Previous articles describing models of ...