Name: in vivo imaging of transgenic leishmania parasites in a live host
Uploaded: 2010-07-27T17:00:00
Description: Watch this Scientific Journal Video about In vivo Imaging of Transgenic Leishmania Parasites in a Live Host at JoVE.com

この作品は、NIHの助成金AI045540、AI067874、AI076233 - 01とAI080801（MEW）、でとAI29646（SMB）で、退役軍人局からメリットレビューの助成金によって一部で賄われていた。作業はNIH T32 AI07511でCTとJGの資金調達時に一部で行われた。

1。トランスジェニックリーシュマニアと小動物の感染症 1。寄生虫行トランスジェニックリーシュマニア属。ルシフェラーゼを発現する寄生虫は、エピソームまたは報告されたとして組み込みベクターを用いて生成されます。1 2クローンの線が好ましい。 2つの重要な点は以下のとおりです。 理論的にはこれら...

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in vivoイメージングシステム（IVIS） で全体の動物のイメージングやリーシュマニア症のさまざまな形態のin vivoイメージングの実験感染モデルにおける方法。18,16 リーシュマニア属を提供します。寄生虫は、IVISイメージング技術による生体内で検出されたレベルでのホタルのルシフェラーゼを発現するように設計することができます。この方?...

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<td>D-Luciferin Potassium Salt </td>
<td>Reagent</td>
<td>Caliper LifeSciences (Formerly Xenogen)</td>
<td>122796</td>
<td>&nbsp;</td>
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<td>IVIS Imaging System 200 Series</td>
<td>Equipment</td>
<td>Caliper LifeSciences</td>
<td>&nbsp;</td>
<td>Other IVIS models that can be used include: Lumina II, Lumina XR, Kinetic, and Spectrum. </td>
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in vivo imaging of transgenic leishmania parasites in a live host

異なる種の<em&gt;リーシュマニア、</em家族の&gt;原生動物の寄生虫<em&gt;トリパノソーマ科</em&gt;、典型的に異なる人間の病気の症状を引き起こす。病気の最も一般的な形態は、内臓リーシュマニア症（VL）および皮膚リーシュマニア症（CL）です。リーシュマニア症のマウスモデルは、広く使われていますが、マウスの病気の間に寄生虫の負担の定量化は、感染後の様々な時点で安楽死するマウスが必要です。寄生虫の負荷は、顕微鏡、希釈アッセイを制限し、または寄生虫のDNAの定量PCRの増幅のいずれかによって測定されます。市販<em&gt; in vivoで</em&gt;イメージングシステム（IVIS）は、生体内の細胞に関連する生物発光シグナルの検出を可能にする統合されたソフトウェアパッケージを持っています。動物の使用を最小限に抑えるために、個人で縦方向に感染に従うために、イメージングのためのin vivoモデルで両方<em&gt;リーシュマニア</em&gt;属。原因となるVLまたはCLが設立された。寄生虫は、ルシフェラーゼを発現するように設計され、これらはどちら皮内又は静脈内マウスに導入した。トランスジェニックのルシフェラーゼ駆動生物発光の定量的測定<em&gt;リーシュマニア</emマウス内の&gt;寄生虫は、IVISを用いて行った。個々のマウスは、私たちは初期の実験的な寄生虫の接種に間動物の変動を評価するため、およびマウス組織における寄生虫の増殖を評価できるように、縦断的研究の間に複数回撮像することができます。寄生虫は皮膚の場所で高感度で検出されています。それは可能性が非常に高いですが、ことは、信号が（寄生虫/フォトン/秒）の皮膚よりも深い内臓器官に低いですが、表面的な対深い部位における信号の定量的な比較は行われていない。それは、同じ組織内の寄生虫の負荷がマウスの間で比較することができるものの、身体部位間の寄生番号を直接、比較することができない可能性があります。 one visceralizing種の例（<em&gt; L. infantum chagasi</em皮膚リーシュマニア症を引き起こす&gt;）と一種（<em&gt; L.メキシカーナ</em&gt;）が表示されます。 IVISの手順は、多種多様の小動物モデルを監視し、分析するために使用することができます<em&gt;リーシュマニア</emヒトリーシュマニア症のさまざまな形態を引き起こす&gt;種。

Watch this Scientific Journal Video about In vivo Imaging of Transgenic Leishmania Parasites in a Live Host at JoVE.com

In vivo Imaging of Transgenic Leishmania Parasites in a Live Host

アン<em&gt; in vivoで</em&gt;イメージングシステムは、トリパノソーマ原虫によるマウス感染の定量的な測定値を生成するために使用されます。<em&gt;リーシュマニア</em&gt;。これは慢性の経過を通して、多くの組織内ルシフェラーゼを発現する寄生虫を検出するための非侵襲的および非致死的方法である<em&gt;リーシュマニア</em&gt;属。感染症。

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Distinct species of Leishmania, a protozoan parasite of the family Trypanosomatidae, typically cause different human disease manifestations. The most ...

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In vivo Imaging of Transgenic Leishmania Parasites in a Live Host

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白血球ホーミングと移行の非侵襲的イメージング in vivoで</em

Two-photon (2P) microscopy is a high resolution imaging technique that has been broadly adapted by biologists.  The value of 2P microscopy is that it ...

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動的な生細胞イメージングのための宿主 - 病原体相互作用の研究のための光トラップの使用

Dynamic live cell imaging allows direct visualization of real-time interactions between cells of the immune system1, 2; however, the lack of spatial and ...

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移植片対宿主病の誘導および<emインビボで&gt;</emMHCマッチしたマウスモデルを使用する&gt; T細胞のモニタリング

Graft-versus-host disease (GVHD) is the limiting barrier to the broad use of bone marrow transplant as a curative therapy for a variety of hematological ...

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The placenta shows a large degree of interspecies anatomic variability. To best understand biology and pathophysiology of the human placenta, it is ...

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細胞内マクロファージの内部成長の定量化は高速とリーシュ マニア原虫の病原性を評価するための信頼性の高い方法です。

The lifecycle of Leishmania, the causative agent of leishmaniasis, alternates between promastigote and amastigote stages inside the insect and vertebrate ...

In Vivo Imaging of Reactive Oxygen Species in a Murine Wound Model

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生体内でマウスにおける活性酸素種のイメージング創モデル

The generation of reactive oxygen species (ROS) is a hallmark of inflammatory processes, but in excess, oxidative stress is widely implicated in various ...

Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium

病原体、共生生物、およびホストの腸上皮間の相互作用を研究する人間 Colonoid 単分子膜

Human 3-dimensional (3D) enteroid or colonoid cultures derived from crypt base stem cells are currently the most advanced ex vivo model of the intestinal ...

In Vivo Infection with Leishmania amazonensis to Evaluate Parasite Virulence in Mice

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マウスの寄生虫毒性を評価するリーシュマニアアマゾネンシスによるVivo感染

Leishmania spp. are protozoan parasites that cause leishmaniases, diseases that present a wide spectrum of clinical manifestations from cutaneous to ...

Live Imaging of Chemokine Receptors in Zebrafish Neutrophils During Wound Responses

創傷応答中のゼブラフィッシュ好中球中のケモカイン受容体のライブイメージング

Leukocyte guidance by chemical gradients is essential for immune responses. Neutrophils are the first cells to be recruited to sites of tissue damage ...

Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice

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Recurrent urinary tract infections (rUTI) caused by uropathogenic Escherichia coli (UPEC) are common and costly. Previous articles describing models of ...