Name: in vivo imaging of transgenic leishmania parasites in a live host
Uploaded: 2010-07-27T17:00:00
Description: Watch this Scientific Journal Video about In vivo Imaging of Transgenic Leishmania Parasites in a Live Host at JoVE.com

Bu çalışma NIH hibe AI045540 AI067874 AI076233-01 ve AI080801 (MEW), ve AI29646 (SMB), Savaş Gazileri İşleri Bölümü Merit İnceleme hibe ile finanse edildi. NIH T32 AI07511 BT ve JG finansman sırasında kısmen yapıldı.

1. Transgenik Leishmania ile küçük hayvanlar enfeksiyon 1. Parazit hatları Transgenik Leishmania spp. lusiferaz ifade parazitler olarak bildirilen bir episomal veya entegre vektör kullanılarak üretilir. 1 2 Klon hatları tercih edilir. İki önemli noktalar şunlardır: Teoride bu parazit çizgiler daha iyi bir memeli içine girmesinden sonra, ilaç basınç, yani yokluğunda transgen korumak beri (a) En...

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In vivo görüntüleme sistemi (IVIS) bütün hayvan görüntüleme için, ya da farklı formlarda leishmaniasis in vivo görüntüleme deneysel enfeksiyon modellerinde bir yöntem sağlar. 18,16 Leishmania spp. parazitler ateşböceği lusiferaz IVIS görüntüleme teknolojisi ile in vivo olarak algılanır bir düzeyde ifade etmek için tasarlanmış olabilir. Bu yöntemin en önemli avantajlarından biri, Leishmania spp non-invaziv görüntüleme sağla...

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<td>D-Luciferin Potassium Salt </td>
<td>Reagent</td>
<td>Caliper LifeSciences (Formerly Xenogen)</td>
<td>122796</td>
<td>&nbsp;</td>
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<td>IVIS Imaging System 200 Series</td>
<td>Equipment</td>
<td>Caliper LifeSciences</td>
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<td>Other IVIS models that can be used include: Lumina II, Lumina XR, Kinetic, and Spectrum. </td>
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in vivo imaging of transgenic leishmania parasites in a live host

Farklı türlerin<em&gt; Leishmania</emAilesinin bir protozoon parazit<em&gt; Trypanosomatidae</em&gt;, Tipik olarak farklı insan hastalık belirtilerine sebep. Hastalığın en yaygın biçimlerinden visseral leishmaniasis (VL) ve kutanöz leishmaniasis (KL). Leishmaniasis&#39;in fare modellerinde yaygın olarak kullanılan, ancak fare hastalık sırasında parazit yüklerin ölçümü fareler enfeksiyondan sonra çeşitli zamanlarda ötenazi gerektirir. Parazit yükler sonra seyreltme parazit DNA testi ya da qPCR amplifikasyon sınırlayıcı, ya mikroskobu ile ölçülür.<em&gt; In vivo</em&gt; Görüntüleme sistemi (IVIS) canlı organizmaların hücreleri ile ilişkili bir bioluminescent sinyal tespiti sağlayan entegre bir yazılım paketi vardır. Hayvan kullanımını en aza indirmek için, bireyler ve görüntüleme için in vivo modellerde boyuna enfeksiyonu takip etmek her ikisi de<em&gt; Leishmania</em&gt; Spp. neden VL veya CL kuruldu. Parazitler lusiferaz ifade etmek için tasarlanmış olup, bunlar ya intradermal veya intravenöz fareler haline getirilmiştir. Transgenik lusiferaz sürüş biyoparlaklık kantitatif ölçümler<em&gt; Leishmania</em&gt; Fare içinde parazitler IVIS kullanılarak yapılmıştır. Ilk deneysel parazit inocula arası hayvan değişimi değerlendirmek amacıyla, fare dokulardaki parazitlerin çarpma değerlendirmek için izin Bireysel fareler, uzunlamasına çalışmalar sırasında birden çok kez görüntülenmiş olabilir. Parazitler kutanöz yerlerde yüksek hassasiyet ile tespit edilir. Sinyali (fotonlar / saniye / parazit) cildin daha derin visseral organlarda düşük, ancak sinyallerin sayısal karşılaştırmalar yüzeysel karşı derin siteler yapılmış olması çok muhtemeldir rağmen. Bu vücut siteler arasında parazit numaraları aynı dokularda parazit yükleri fareler arasında karşılaştırıldığında olmasına rağmen doğrudan karşılaştırıldı olamaz ki mümkündür. Bir visceralizing türün örnekleri (<em&gt; L. infantum chagasi</emKutanöz leishmaniasis neden&gt;) ve tek bir tür (<em&gt; L. mexicana</em&gt;) Gösterilmiştir. IVIS prosedürü geniş bir yelpazede küçük hayvan modellerinde izleme ve analiz için kullanılabilir<em&gt; Leishmania</em&gt; Insan leishmaniasis farklı formları neden türleri.

Watch this Scientific Journal Video about In vivo Imaging of Transgenic Leishmania Parasites in a Live Host at JoVE.com

In vivo Imaging of Transgenic Leishmania Parasites in a Live Host

Bir<em&gt; In vivo</em&gt; Görüntüleme sistemi Trypanosomatid protozoon ile fare enfeksiyon kantitatif ölçümler oluşturmak için kullanılır<em&gt; Leishmania</em&gt;. Bu kronik seyri boyunca birçok dokuda içinde lusiferaz ifade parazitleri tespit etmek için bir non-invaziv ve öldürücü olmayan bir yöntemdir<em&gt; Leishmania</em&gt; Spp. enfeksiyon.

Canlı bir Host Transgenik Leishmania Parazitlerinin in vivo Imaging

Distinct species of Leishmania, a protozoan parasite of the family Trypanosomatidae, typically cause different human disease manifestations. The most ...

Research

JoVE Journal

Immunology and Infection

In vivo Imaging of Transgenic Leishmania Parasites in a Live Host

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Hızlı ve güvenilir yöntem LEISHMANIA parazit virülans değerlendirmek için hücre içi büyüme makrofajlar içinde miktar olduğunu

The lifecycle of Leishmania, the causative agent of leishmaniasis, alternates between promastigote and amastigote stages inside the insect and vertebrate ...

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Human 3-dimensional (3D) enteroid or colonoid cultures derived from crypt base stem cells are currently the most advanced ex vivo model of the intestinal ...

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Leishmania spp. are protozoan parasites that cause leishmaniases, diseases that present a wide spectrum of clinical manifestations from cutaneous to ...

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Yara Yanıtları Sırasında Zebra Balığı Nötrofillerdeki Kemokin Reseptörlerinin Canlı Görüntülenmesi

Leukocyte guidance by chemical gradients is essential for immune responses. Neutrophils are the first cells to be recruited to sites of tissue damage ...

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