Name: orthotopic aortic transplantation: a rat model to study the development of chronic vasculopathy
Uploaded: 2010-12-04T17:00:00
Description: Watch this Scientific Journal Video about Orthotopic Aortic Transplantation: A Rat Model to Study the Development of Chronic Vasculopathy at JoVE.com

作者感谢她的组织学工作的克里斯蒂安Pahrmann。 资助宋佳Schrepfer已收到由德意志研究联合会（DFG）（SCHR992 / 3 1和SCHR992/4-1）的研究补助金。]

查尔斯河（Sandhofer WEG 7，D - 97633 Sulzfeld）均购自大鼠，体重约250 - 300G。 使用PVG大鼠作为捐助者，和作为接受者的ACI大鼠移植手术。 老鼠被安置在常规条件下，美联储标准大鼠议员和水广告libidum。 所有手术器械消毒后才能使用。 捐助者的准备： 与异氟醚麻醉大鼠（2,5-3％）使用感应室。 <ol><li>剃须的腹部和?...

<ol>
	<li>Deuse, T., Hoyt, G., Koyanagi, T., Robbins, R. C., Schrepfer, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prevention+and+Inhibition+But+Not+Reversion+of+Chronic+Allograft+Vasculopathy+by+FK778.">Prevention and Inhibition But Not Reversion of Chronic Allograft Vasculopathy by FK778.</a> Transplantation. 85, 870-877 (2008).</li><li>Mennander, A., Tiisala, S., Halttunen, J., Yilmaz, S., Paavonen, T., Hayry, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chronic+rejection+in+rat+allografts.+An+experimental+model+for+transplant+arteriosclerosis.">Chronic rejection in rat allografts. An experimental model for transplant arteriosclerosis.</a> Arterioscler Thromb Vasc Biol. 11, 671-680 (1991).</li><li>Ono, K., Lindsey, E. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Improved+technique+of+heart+transplantation+in+rats.">Improved technique of heart transplantation in rats.</a> J Thorac Cardiovasc Surg. 57, 225-225 (1969).</li><li>Poston, R. S., Billingham, M., Hoyt, E. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rapamycin+reverses+chronic+graft+vascular+disease+in+a+novel+cardiac+allograft+model.">Rapamycin reverses chronic graft vascular disease in a novel cardiac allograft model.</a> Circulation. 100, 67-100 (1999).</li><li>Schrepfer, S., Deuse, T., Koch-Nolte, F., Krieger, T., Haddad, M., Schäfer, H., Pelletier, M. P., Robbins, R. C., Reichenspurner, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=FK778+in+Experimental+Xenotransplantation:+A+Detailed+Analysis+of+Drug+Efficacy.">FK778 in Experimental Xenotransplantation: A Detailed Analysis of Drug Efficacy.</a> J Heart Lung Transplant. 26, 70-77 (2007).</li></ol>

由于一些原因，我们发现主动脉原位移植模型，更准确的方式来探讨异位心脏移植模型相比，TVP发展： 主动脉移植模型的手术技术方面，体现的是一种方便可行的方法，只需要在月底到年底，吻合的成就，而异位心脏移植模型可能被视为一个更加复杂和容易出错的外科医生的技能挑战，需要在两个主要血管端至端侧吻合。 组织学标本的分析和测量大幅促进移植血管病变?...

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<td>Surgical microscope (magnification 25x)</td>
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<td>ice cold 0.9% saline</td>
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<td>prewarmed 0.9% sterile saleine</td>
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orthotopic aortic transplantation: a rat model to study the development of chronic vasculopathy

慢性排斥反应的研究模型是必不可少的调查期间移植血管病变（TVP）的发展的病理生物学和病理生理过程。 心血管慢性排斥反应的研究中常用的动物模型是在啮齿类实验动物进行异位心脏移植模型。这种模式是在实验中广泛使用，因为小野和Lindsey（3）发表了他们的的技术。要分析的结果，在血管，心，切片，所有船只都必须测量。 调查心血管questionings慢性排斥反应的另一种方法是主动脉移植模型（1，2）。主动脉主动脉原位移植模型，可以很容易地组织评估（2）。 PVG -预报模式是CAV的研究特别有用，因为急性血管性排斥反应的主要混杂因素和环孢素A（CsA）治疗并不妨碍CAV的发展，我们在临床上发现类似（4 ）。 A7天的环孢素A的时期，是需要在此模型中，以防止急性排斥反应，并实现长期生存与发展的TVP。 这种模式也可以用来研究急性细胞排斥反应和异种模型（5）媒体坏死。

Watch this Scientific Journal Video about Orthotopic Aortic Transplantation: A Rat Model to Study the Development of Chronic Vasculopathy at JoVE.com

Orthotopic Aortic Transplantation: A Rat Model to Study the Development of Chronic Vasculopathy

本视频演示了一个简单的模型来研究在大鼠体内的移植血管病变（TVP）的发展主动脉原位移植模型。

主动脉原位移植大鼠模型，研究慢性血管病变的发展

Research models of chronic rejection are essential to investigate pathobiological and pathophysiological processes during the development of transplant ...

Research

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Human Internal Mammary Artery (IMA) Transplantation and Stenting: A Human Model to Study the Development of In-Stent Restenosis

Human Internal Mammary Artery IMA Transplantation and Stenting: A Human Model to Study the Development of In-Stent Restenosis

人乳内动脉（IMA）移植及内支架置入术：人体模型支架内再狭窄的研究开发

Preclinical in vivo research models to investigate pathobiological and pathophysiological processes in the development of intimal hyperplasia after vessel ...

科研

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Development of Obliterative Bronchiolitis in a Murine Model of Orthotopic Lung Transplantation

闭塞性细支气管炎在原位肺移植小鼠模型的发展

Orthotopic lung transplantation in rats was first reported by Asimacopoulos and colleagues in 1971 1. Currently, this method is well accepted and ...

Orthotopic Aortic Transplantation in Mice for the Study of Vascular Disease

原位主动脉瓣移植在小鼠血管疾病的研究

Vascular procedures involving anastomoses in the mouse are generally thought to be difficult and highly dependent on the skill of the individual surgeon. ...

Surgical Procedures for a Rat Model of Partial Orthotopic Liver Transplantation with Hepatic Arterial Reconstruction

部分原位肝移植大鼠模型的肝动脉重建的外科手术

Orthotopic liver transplantation (OLT) in rats using a whole or partial graft is an indispensable experimental model for transplantation research, such as ...

A Model of Chronic Nutrient Infusion in the Rat

在大鼠模型慢性营养输液

Chronic exposure to excessive levels of nutrients is postulated to  affect the function of several organs and tissues and to contribute to the  ...

Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation

小鼠角膜移植模型，研究实体器官移植的最常见形式

Corneal transplantation is the most common form of organ transplantation in the United States with between 45,000 and 55,000 procedures performed each ...

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

用线 Myography 测定小鼠肠系膜动脉的等轴收缩力

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...

A Pre-Clinical Porcine Model of Orthotopic Heart Transplantation

正交心脏移植的临床前猪模型

Fifty-years following the first successful report, cardiac transplantation remains the gold-standard treatment for eligible patients with advanced heart ...

Orthotopic Rat Kidney Transplantation: A Novel and Simplified Surgical Approach

大鼠原位肾移植: 一种新的简化的手术方法

Kidney transplantation offers increased survival rates and improved quality of life for patients with end-stage renal disease, as compared to any type of ...

Reduced Complications after Arterial Reconnection in a Rat Model of Orthotopic Liver Transplantation

矫形肝移植大鼠模型中动脉重联后并发症减少

The rat orthotopic liver transplantation (OLT) model is a powerful tool to study acute and chronic rejection. However, it is not a complete representation ...