June 26th, 2010
•This protocol describes a general approach to perform photoconversion of fluorescent proteins on a confocal laser scanning microscope. We describe procedures for the photoconversion of puried protein samples, as well as for dual-probe optical highlighting in live cells with mOrange2 and Dronpa.
Tags
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved