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DOI :
10.3791/1996-v
•
7:38 min
June 16th, 2010
Chapters
0:00
Title
0:50
Introduction
1:26
Preparation and Staining of Sperm Cells
4:32
Imaging Sperm Intracellular Ca Signals
7:06
Conclusion
6:20
Single-cell Fluorescence Intensity Data and its Relationship to Free Calcium
刺激诱发的[Ca 2 +]:我个人的人类精子的信号进行评估。运动型细胞加载与Ca 2 +敏感的荧光染料(AM -酯法),并固定在perfusable室。时间推移荧光显微镜和细胞成像,通过灌注介质刺激。单细胞的反应(或地区)使用Excel进行离线分析。
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