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03:20 min
April 28th, 2023
DOI :
10.3791/200046-v
Transcript
根据制造商的说明准备高压冷冻或HPF机器,至少在开始实验前两个小时。将A型HPF铝载体转移到含有1毫升乙醇的2毫米离心管中,并在超声波清洗机中超声处理10分钟。在覆盖有定性滤纸的培养皿中干燥载体。
将预先清洁的载体浸泡在1-十六碳烯中。使用细镊子从HeLa细胞培养皿中取出预先准备好的蓝宝石盘。用定性滤纸触摸标记的表面以去除多余的介质。
将蓝宝石圆盘安装到 HPF 样品支架中,并用含有 1-十六烯的 0.025 毫米深铝载体快速盖住圆盘。用定性滤纸吸出过量溶液。装载试
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