National Institute of Biological Sciences, Beijing

3 ARTICLES PUBLISHED IN JoVE

Developmental Biology

A Standardized Method for Measuring Internal Lung Surface Area via Mouse Pneumonectomy and Prosthesis Implantation

¹Department of Life Sciences, Peking University, ²National Institute of Biological Sciences, Beijing, ³Graduate School of Peking Union Medical College

Internal lung surface area (ISA) is a critical criterion for assessing lung morphology and physiology in lung diseases and injury-induced alveolar regeneration. We describe here a standardized method that can minimize the measurement bias for ISA in both lung pneumonectomy and prosthesis implantation mouse models.

Neuroscience

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

Long Mei^1,2, Cheng Zhan², Eric Erquan Zhang²

¹PTN Joint Graduate Program, School of Life Sciences, Peking University, ²National Institute of Biological Sciences, Beijing

This newly developed fluorescence-based technology enables long-term monitoring of the transcription of circadian clock genes in the suprachiasmatic nucleus (SCN) of freely moving mice in real-time and at a high temporal resolution.

Biology

Direct Synthesis of EM-Visible Gold Nanoparticles in Cells for Protein Localization Analysis with Well-Preserved Ultrastructure

Zhaodi Jiang^1,2

¹National Institute of Biological Sciences, Beijing, ²Tsinghua Institute of Multidisciplinary Biomedical Research, Tsinghua University

The present protocol describes a clonable electron microscopy labeling technology for detecting metallothionein-tagged proteins in cells using a novel autonucleation suppression mechanism-based gold nanoparticle synthesis technique.