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03:23 min
April 28th, 2023
DOI :
10.3791/200047-v
Transcript
取预先准备好的含有稳定HeLa细胞的HPF和FSF处理的蓝宝石盘,用PBS-A缓冲液洗涤五分钟,然后重复此过程三次。在室温下将蓝宝石盘转移到含有一毫升PBS-A缓冲液的35毫米培养皿中。用一毫升还原溶液替换培养皿中的PBS-A缓冲液,并在室温下孵育一小时。
在一毫升还原溶液中制备金前体,并立即涡旋混合。在金前体溶液中加入80微升500毫摩尔D-青霉胺的双蒸水中。并立即涡旋。
用一毫升金前体溶液替换培养皿中的溶液,并在四摄氏度下孵育两小时。在金前驱体溶液中加入20至1
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