Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
TR - Turkish
JA - Japanese
Please note that all translations are automatically generated. Click here for the English version.
106 Views
•
01:42 min
June 23rd, 2023
DOI :
10.3791/200183-v
Transcript
从含有电穿孔罗伊氏乳杆菌细胞的MRS琼脂平板中挑选任何菌落,并接种到含有每孔过滤灭菌MRS肉汤和适当选择抗生素的96孔微孔板中。在 37 摄氏度下有氧孵育 24 小时,不要摇晃。第二天,罗伊氏乳杆菌将在固定相中沉淀出培养基。
通过移液重悬培养物。将 200 微升培养物加入平坦、透明的底部 96 孔板中。将板转移到读板器上,测量M樱桃二号和其他相关报告基因的光密度和荧光。
在氧气存在下,菌落形态发生明显变化。在低氧条件下,菌落呈白色、不透明、光滑和圆形。在部分或渗漏的
Sign in or start your free trial to access this content
Explore More Videos
From the series
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Librarians
Access
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved