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Please note that all translations are automatically generated. Click here for the English version.
106 Views
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03:14 min
June 16th, 2023
DOI :
10.3791/200234-v
* These authors contributed equally
Transcript
首先,将麻醉的小鼠放在手术平台上。修剪头发后,在双眼上涂抹眼药膏,以防止它们干燥。然后将小鼠固定在立体定位装置上进行固定。
在显微镜下做一个小切口,使颅骨完全暴露。准备两个 10 微升注射器,一个含有 AAV2/5-腺苷 2A 受体 shRNA,另一个含有 AAV2/5 加扰剂。然后使用显微镜定位前囟和λ,并基于小鼠大脑立体定位图谱在10微升注射器上设置坐标点。
在已建立的坐标点处在头骨上钻一个小孔。通过侧向注射以每分钟100纳升的稳定速率将两微升AAV2 / 5-
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