The Second Affiliated Hospital of Wenzhou Medical University

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity

Shen Zhang¹, Yonggen Wu¹, Yimin Weng², Zhihui Xu¹, Wenmin Chen³, Dahan Zheng⁴, Wei Lin⁵, Jun Liu⁶, Ying Zhou^1,7

¹Reproductive Medicine Center, The First Affiliated Hospital of Wenzhou Medical University, ²Department of Orthopaedics, The Second Affiliated Hospital of Wenzhou Medical University, ³Department of Obstetrics, The First Affiliated Hospital of Wenzhou Medical University, ⁴School of Laboratory Medicine and Life Science, Wenzhou Medical University, ⁵School of Pharmaceutical Science, Wenzhou Medical University, ⁶Stem Cells and Genetic Engineering Group, AgriBioscience Research Centre, Department of Economic Development, Jobs, Transport and Resources, ⁷Department of Histology and Embryology, Wenzhou Medical University

A highly promising technique to generate tissue constructs without using matrix is to culture cells in a simulated microgravity condition. Using a rotary culture system, we examined ovarian follicle growth and oocyte maturation in terms of follicle survival, morphology, growth, and oocyte function under the simulated microgravity condition.

Neuroscience

Targeted Knockdown of Genes in the Choroid Plexus

Yuwen Yang^*1, Chenxing Qi^*1, Lanxin Hu¹, Cheng Zheng¹, Xuhang Li¹, Wu Zheng¹, Yiyun Weng², Haiyan Lin^3,4

¹State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital, Wenzhou Medical University, ²Department of Neurology, the First Affiliated Hospital of Wenzhou Medical University, ³Rehabilitation Medicine Center, The Second Affiliated Hospital of Wenzhou Medical University, ⁴Integrative & Optimized Medicine Research Center, China-USA Institute for Acupuncture and Rehabilitation, Wenzhou Medical University

Here, we describe a method to selectively alter gene expressions in the choroid plexus while avoiding any impact in other brain areas.