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Visualizing Recombinant Protein-Filled Vesicles from E. coli by Fluorescence Microscopy (Video) | JoVE

DOI :

10.3791/200249-v

•

1:40 min

•

June 30th, 2023

June 30th, 2023

•
256 Views
1School of Biosciences, University of Kent

Tags

Visualizing
Recombinant Protein filled Vesicles
E Coli
Fluorescence Microscopy
Staining
Fluorescent Lipid Dye
FM4 64
Purified Vesicles
Incubate
Imaging
Isolation
Pipette
Agarose Pad
Glass Slide
Cover Slip
Spacers
Adhesive Tape
Inverted Microscope
Oil Immersion Objective
Settling
Equilibrate
Image Averaging
Random Background Noise Reduction
Time lapse Imaging
Widefield Fluorescence Microscopy
Amnion Green containing Vesicles
FM4 64 Dye Confirmation
Structured Illumination Microscopy
Live Bacterial Cells
Inner Membrane Protein Expression
Cargo Insertion

-- Views

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Visualizing Recombinant Protein-Filled Vesicles from E. coli by Fluorescence Microscopy

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