Name: patterned photostimulation with digital micromirror devices to investigate dendritic integration across branch points
Uploaded: 2011-03-02T17:30:00
Description: Watch this Scientific Journal Video about Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points at JoVE.com

<p class="jove_content">这项工作是从美国国立卫生研究院和优异评论RO1的C. - MT从弗吉尼亚州的研究服务，支持和个人NRSA到CWL </p>

<p class="jove_title">一般的设计考虑</p><p class="jove_content">如果照片刺激的波长在可见光范围内，如optogenetic实验，的，系统的布局，将大大高于简单紫外光解实验。人们只需要购买一个双摄像头端口模块，显微镜公司所有。 DMD的平面，可以放置在任何一个的两个摄像头端口的共轭平面图像。但在紫外线光解从DMD的光必须被通过的外延照明路径（图3A），因为相机的成像管镜头是没有任何市售显微镜紫外线波长纠正。在...

<ol>
	<li>Scanziani, M., Hausser, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electrophysiology+in+the+age+of+light.">Electrophysiology in the age of light.</a> <em style='font-style: italic'>Nature</em>. <b>461</b>, 930-939 (2009).</li><li>Tang, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Photolysis+of+caged+neurotransmitters:+Theory+and+procedures+for+light+delivery.">Photolysis of caged neurotransmitters: Theory and procedures for light delivery.</a> <em style='font-style: italic'>Curr. Prot. Neurosci</em>. , 6.21.1-6.21.12 (2006).</li><li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nature+Technology+Feature,+Cell+imaging:+Light+activated.">Nature Technology Feature, Cell imaging: Light activated.</a> <em style='font-style: italic'>Nature</em>. <b>456</b>, 826-827 (2008).</li><li>Lutz, C., Otis, T. S., DeSars, V., Charpak, S., DeGregorio, D. A., Emiliani, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Holographic+photolysis+of+caged+neurotransmitters.">Holographic photolysis of caged neurotransmitters.</a> <em style='font-style: italic'>Nature Methods</em>. <b>5</b>, 821-827 (2008).</li><li>Hornbeck, L. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> <em style='font-style: italic'>Digital Light Processing and MEMs: An overview</em>. , .</li></ol>

<p class="jove_content">基于DMD的photostimulation方法的优点是最明显的目标中占有相对大面积的情况下。如果利益的目标是非常小的，如几个树突棘，的，连续扫描共聚焦和双光子系统可能是更好的方法。 DMD的方法的一个重大弱点是其低效利用现有的光。大部分可用的光必然是向关镜，不使用。 </p><p class="jove_content">基于DMD的制度是最适合在可见光范围内操作。我们期望与光遗传学实验时，DMD的基础photostimulation系统将作?...

Erratum: Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points

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<td>Modern upright fluorescent microscope</td>
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<td>Computer and data acquisition/interface system</td>
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<td>DLP Discovery Developer Kit </td>
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<td>ALP3 USB interface</td>
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<td>S2 + Optics w/LED</td>
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<td>Dual camera port unit</td>
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<td>355nm frequency tripled NdVO4 laser (~1 W)</td>
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<td>DPSS Laser Inc.</td>
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<td>Laser shutter Model LS6</td>
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<td>Uniblitz</td>
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<td>Multimode optical fiber and fiber stretcher Model# 915</td>
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<td>Canadian Instrument and Research, Ltd </td>
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<td>100 um core multimode fiber</td>
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<td>Multimode Fiber launcher </td>
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<td>Oz Optics</td>
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<td>Signal generator</td>
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<td>up to 50 kHz</td>
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<td>Beam collimator</td>
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<td>Olympus</td>
<td>DApo20UV340</td>
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<td>UV relay lens</td>
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patterned photostimulation with digital micromirror devices to investigate dendritic integration across branch points

<p class="jove_content"> Light是一个通用和精确的手段来控制神经元的兴奋性。近期出台的通道的视紫红质和笼中的神经递质，如光敏感效应，导致以利益在开发更好的手段来控制光的模式，在空间和时间是有用的实验神经科学。一个传统的战略，在共聚焦和双光子显微镜就业，是集中光线的衍射有限点，并在感兴趣的区域，然后扫描顺序单点。这种方法就出现了问题，如果大面积必须在很短的时间窗口，一个问题比成像更适用photostimulation的刺激。另一种方法是数字微镜器件（DMD）的援助项目的完成对目标的空间格局。 DMD的做法是有吸引力，因为硬件组件都相对便宜，而且是由商业利益的支持。因为这种制度是不是直立式显微镜，我们将讨论在这样一个DMD系统的建设和运营的关键问题。即使我们将主要描述紫外光解的制度建设，为建设optogenetic实验可见光系统简单得多的修改也将提供。紫外光解系统是用于结转库存实验，以研究在神经科学中的一个根本性的问题，如何在空间上分布在远端树突分支点综合投入。结果表明，一体化非线性跨分支点和supralinearity主要是由NMDA受体介导的。 </p>

Watch this Scientific Journal Video about Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points at JoVE.com

Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points

<p class="jove_content">数字微镜器件（DMD），可以在时间和空间产生复杂的图案，用以控制神经元的兴奋性。的DMD系统的设计，施工，操作的有关问题进行了讨论。这样一个系统，使在远端树突分支点非线性一体化的示范。</p>

图案与数字微镜装置Photostimulation调查跨分支点的树突状融合

Light is a versatile and precise means to control neuronal excitability. The recent introduction of light sensitive effectors such as channel-rhodopsin ...

Research

JoVE Journal

Bioengineering

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement

蛋白质和细胞坐月子创建二维图案衬底

Microcontact printing provides a rapid, highly reproducible method for the creation of well-defined patterned substrates.1  While microcontact printing ...

科研

生物工程

Design of a Cyclic Pressure Bioreactor for the <em>Ex Vivo</em> Study of Aortic Heart Valves

Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves

循环压力为生物反应器的设计<em>体外</em>主动脉心脏瓣膜的研究

The aortic valve, located between the left ventricle and the aorta, allows for unidirectional blood flow, preventing backflow into the ventricle. Aortic ...

Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers

模型和方法来评价药物输送系统在整个细胞屏障的运输

Sub-micrometer carriers (nanocarriers; NCs) enhance efficacy of drugs by improving solubility, stability, circulation time, targeting, and release. ...

Helical Organization of Blood Coagulation Factor VIII on Lipid Nanotubes

凝血因子VIII的脂质纳米管螺旋组织

Cryo-electron microscopy (Cryo-EM)1 is a powerful approach to investigate the functional structure of proteins and complexes in a hydrated state and ...

Planar Gradient Diffusion System to Investigate Chemotaxis in a 3D Collagen Matrix

平面梯度扩散系统的调查趋化三维胶原基质

The importance of cell migration can be seen through the development of human life. When cells migrate, they generate forces and transfer these forces to ...

Fabrication of a Functionalized Magnetic Bacterial Nanocellulose with Iron Oxide Nanoparticles

一机能磁性纳米纤维素的细菌与氧化铁纳米粒子的制备

In this study, bacterial nanocellulose (BNC) produced by the bacteria Gluconacetobacter xylinus is synthesized and impregnated in situ with iron oxide ...

Fabricating Cotton Analytical Devices

制造棉分析仪器

A robust, low-cost analytical device should be user-friendly, rapid, and affordable. Such devices should also be able to operate with scarce samples and ...

Using Adhesive Patterning to Construct 3D Paper Microfluidic Devices

使用粘性图案构建3D纸微流体装置

We demonstrate the use of patterned aerosol adhesives to construct both planar and nonplanar 3D paper microfluidic devices. By spraying an aerosol ...

An Experimental and Finite Element Protocol to Investigate the Transport of Neutral and Charged Solutes across Articular Cartilage

实验和有限元议定书调查中性和带电溶质的跨关节软骨运输

Osteoarthritis (OA) is a debilitating disease that is associated with degeneration of articular cartilage and subchondral bone. Degeneration of articular ...

Soft Lithographic Procedure for Producing Plastic Microfluidic Devices with View-ports Transparent to Visible and Infrared Light

生产塑料微流控装置与查看端口对可见光和红外透明软光刻过程

Infrared (IR) spectro-microscopy of living biological samples is hampered by the absorption of water in the mid-IR range and by the lack of suitable ...