Name: Establishment of Murine Chronic Root Ganglion Compression Model
Uploaded: 2023-07-14T13:50:00
Description: Watch this Scientific Journal Video about Establishment of Murine Chronic Root Ganglion Compression Model at JoVE.com

establishment of murine chronic root ganglion compression model

Protocolo Tuina Padronizado para Modelo de Compactação DRG de Rato

In clinical settings, it is common to observe neurological pathological pain caused by nerve root compression due to various reasons. The most typical form of this neuropathic pain is lumbar disc herniation (LDH), which is often persistent, recurrent, and difficult to cure. Approximately 9% of the global population is affected by LDH, leading to significant social and economic burdens1. The incidence of this type of neuropathic pain is increasing yearly, with a trend toward younger patients, due to changes in human production and lifestyle2. Despite the use of non-steroidal painkillers, patients&#39; symptoms cannot be completely alleviated. As a result, alternative therapies, such as Tuina, for treating pain caused by LDH have gained increasing attention.
Tuina therapy, a form of conservative treatment for LDH, is widely recommended in various clinical practice guidelines worldwide for preventing and treating lower back pain3,4. Research has shown that Tuina can significantly lower inflammatory factors such as serum IL-6 and tumor necrosis factor-alpha (TNF-&#945;) levels in LDH patients while improving patients&#39; pain and lumbar function impairment5. However, the specific mechanism behind Tuina therapy&#39;s pain-relieving effects remains unclear.
Animal models are a valuable tool for studying neuropathic pain caused by LDH6. They allow for behavioral measurements to evaluate the effectiveness of Tuina therapy and provide samples of the pathological physiology of LDH. For example, samples from the dorsal root ganglia in the thigh can be taken to verify changes in dorsal root ganglion cells. The chronic compression of the Dorsal Root Ganglion (CCD) model is commonly used to evaluate the pathological physiology of LDH, as it causes damage to the morphology of dorsal root ganglion cells that are consistent with the pathological changes seen in clinical cases of nerve compression caused by disc herniation7.
Many scholars have conducted several animal experiments on acupressure analgesia8,9,10. However, when implementing acupressure operations on animal models, they often imitate human acupressure. The therapeutic effect of acupressure is affected by factors such as the size, frequency, and the direction of the applied force11,12,13. If the experiment lacks a unified acupressure standard, such as the force, frequency, and duration of the operation, this may cause some deviation in the experimental results. This article introduces a set of acupressure treatment plans based on the characteristics of CCD rats, and promotes the development of standardized acupressure operations in animal models.

Autor em destaque: Efeito analgésico da Tuina em modelos de ratos com compressão da dor do gânglio da raiz dorsal  

Efeito Analgésico de Tuina em Modelos de Rato com Compressão da Dor do Gânglio da Raiz Dorsal

Tuina analgesia is widely used in clinical practice. Our research focuses on the mechanism of action of Tuina analgesia, and investigate how Tuina achieve its analgesic effect. Achieving standardization in Tuina is currently the most significant obstacle.The effectiveness of Tuina is influenced by several factors such as treatment, area type, intensity, and frequency. The current protocol is optimized in two aspect based on the earlier research. Firstly, the force and frequency of pushing and holding operations were standardized and controlled by force-measuring gloves.Secondly, the results of imaging and animal behavior test were integrated to assess the results of modeling.

Watch this Scientific Journal Video about Establishment of Murine Chronic Root Ganglion Compression Model at JoVE.com

Establishment of Murine Chronic Root Ganglion Compression Model  

Estabelecimento do Modelo de Compressão do Gânglio Radicular Crônico Murino

Para estabelecer o modelo animal, comece fixando um rato anestesiado em uma placa de espuma, prendendo seus membros e incisivos usando elásticos. Limpe a área preparada com um cotonete com álcool a 75%. Em seguida, use uma tesoura para fazer uma incisão de dois a três centímetros através da pele, fáscia superficial e fáscia profunda camada por camada.Localizar a espinha ilíaca anterossuperior, seguida do terceiro e quarto processos espinhosos. Aperte o processo espinhoso com pinça dentada e levante-o para permitir que a tesoura fique próxima ao lado direito do processo espinhoso. Corte o músculo preso ao lado direito do processo espinhoso.Em seguida, disseque bruscamente o músculo aderido à superfície externa da placa vertebral até que a resistência seja sentida. Da mesma forma, dissecar bruscamente o músculo e a fáscia na articulação zigapofisial. Use uma sonda em forma de L para localizar o forame intervertebral.Em seguida, insira uma haste de aço inoxidável em forma de L no quarto e quinto forame intervertebral lombar. Proceder à sutura do músculo, fáscia e pele camada por camada usando pontos 3-0. Coloque o rato em uma caixa termostática até que ele acorde e observe a função do membro posterior direito do rato para garantir a normalidade.

Research

JoVE Journal

Medicine

Establishment of Murine Chronic Root Ganglion Compression Model

Analgesic Effect of Tuina on Rat Models with Compression of the Dorsal Root Ganglion Pain

Neuropathic pain is a prevalent condition that affects 6.9%-10% of the population and results from nerve damage due to various etiologies, such as lumbar ...