Name: Gedragstesten bij muizenpijn om het effect van Tuina-therapie te bestuderen
Uploaded: 2023-07-14T13:50:00
Description: Watch this Scientific Journal Video about Murine Pain Behavioral Testing to Study the Effect of Tuina Therapy at JoVE.com

murine pain behavioral testing to study the effect of tuina therapy

Gestandaardiseerd Tuina-protocol voor DRG-compressiemodel voor ratten

In clinical settings, it is common to observe neurological pathological pain caused by nerve root compression due to various reasons. The most typical form of this neuropathic pain is lumbar disc herniation (LDH), which is often persistent, recurrent, and difficult to cure. Approximately 9% of the global population is affected by LDH, leading to significant social and economic burdens1. The incidence of this type of neuropathic pain is increasing yearly, with a trend toward younger patients, due to changes in human production and lifestyle2. Despite the use of non-steroidal painkillers, patients&#39; symptoms cannot be completely alleviated. As a result, alternative therapies, such as Tuina, for treating pain caused by LDH have gained increasing attention.
Tuina therapy, a form of conservative treatment for LDH, is widely recommended in various clinical practice guidelines worldwide for preventing and treating lower back pain3,4. Research has shown that Tuina can significantly lower inflammatory factors such as serum IL-6 and tumor necrosis factor-alpha (TNF-&#945;) levels in LDH patients while improving patients&#39; pain and lumbar function impairment5. However, the specific mechanism behind Tuina therapy&#39;s pain-relieving effects remains unclear.
Animal models are a valuable tool for studying neuropathic pain caused by LDH6. They allow for behavioral measurements to evaluate the effectiveness of Tuina therapy and provide samples of the pathological physiology of LDH. For example, samples from the dorsal root ganglia in the thigh can be taken to verify changes in dorsal root ganglion cells. The chronic compression of the Dorsal Root Ganglion (CCD) model is commonly used to evaluate the pathological physiology of LDH, as it causes damage to the morphology of dorsal root ganglion cells that are consistent with the pathological changes seen in clinical cases of nerve compression caused by disc herniation7.
Many scholars have conducted several animal experiments on acupressure analgesia8,9,10. However, when implementing acupressure operations on animal models, they often imitate human acupressure. The therapeutic effect of acupressure is affected by factors such as the size, frequency, and the direction of the applied force11,12,13. If the experiment lacks a unified acupressure standard, such as the force, frequency, and duration of the operation, this may cause some deviation in the experimental results. This article introduces a set of acupressure treatment plans based on the characteristics of CCD rats, and promotes the development of standardized acupressure operations in animal models.

Auteur in de schijnwerpers: Pijnstillend effect van Tuina op rattenmodellen met compressie van de dorsale wortel Ganglionpijn  

Pijnstillend effect van Tuina op rattenmodellen met compressie van de dorsale wortel Ganglionpijn

Tuina analgesia is widely used in clinical practice. Our research focuses on the mechanism of action of Tuina analgesia, and investigate how Tuina achieve its analgesic effect. Achieving standardization in Tuina is currently the most significant obstacle.The effectiveness of Tuina is influenced by several factors such as treatment, area type, intensity, and frequency. The current protocol is optimized in two aspect based on the earlier research. Firstly, the force and frequency of pushing and holding operations were standardized and controlled by force-measuring gloves.Secondly, the results of imaging and animal behavior test were integrated to assess the results of modeling.

Watch this Scientific Journal Video about Murine Pain Behavioral Testing to Study the Effect of Tuina Therapy at JoVE.com

Gedragstesten bij muizenpijn om het effect van Tuina-therapie te bestuderen

Om de Von Fray-test uit te voeren, begint u met het plaatsen van de ratten in een transparant compartiment van gehard glas op een metalen draadrooster op een hoogte van 40 centimeter. Meet vervolgens de mechanische terugtrekkingsdrempel met elektronische vezels van Von Frey. Stimuleer de voet van de rat totdat deze merkbaar reageert, bijvoorbeeld door de poot op te tillen of te ontwijken.Om de latentie van het terugtrekken van de poot te beoordelen, of PWL met behulp van de Hargreaves-methode, richt u de schijnwerper op het midden van de voet van de rat en drukt u op de startknop. Houd de rat in de gaten op gedrag zoals het terugtrekken van de voet of het likken van de poten en druk nogmaals op de startknop om de tijd vast te leggen. Voer de test drie keer achter elkaar uit op dezelfde achterpoot.Na 17 dagen Tuina-therapie werd een significant verschil waargenomen in de drempels voor het terugtrekken van de poten tussen de CCD-ratten die Tuina-therapie kregen en de onbehandelde CCD-groep. De ratten in de CCD-groep die Tuina-therapie kregen, vertoonden vanaf het begin van de behandeling verbetering van de pijngrens. Vanaf dag 14 verschilden de thermische pijndrempels significant tussen de CCD-groep en de Tuina-groep.

Research

JoVE Journal

Medicine

Murine Pain Behavioral Testing to Study the Effect of Tuina Therapy

Analgesic Effect of Tuina on Rat Models with Compression of the Dorsal Root Ganglion Pain

Neuropathic pain is a prevalent condition that affects 6.9%-10% of the population and results from nerve damage due to various etiologies, such as lumbar ...