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Transfer and Rearing of Butterfly Eggs on Agar-Based Diet Until Adult Emergence (Video) | JoVE

DOI :

10.3791/200339-v

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4:00 min

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August 18th, 2023

August 18th, 2023

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144 Views

Emilie C. Snell-Rood¹, Megan E. Kobiela^1,2

¹Deptartment of Ecology, Evolution and Behavior, University of Minnesota, ²Biology, Sweet Briar College

Tags

Artificial Diet Preparation Recipe Ingredients 15 G Fine Mesh Agar 400 Ml Distilled Water 400 Ml Faucet temperature Distilled Water 5 Ml Flaxseed Oil Instructions 1 Weigh All The Dry Ingredients

Into One Container 2 Place The Dry Ingredient Mixture Into A Blender And Add 5 Ml Of Flaxseed Oil 3 In A 1 liter Beaker

Mix 15 G Of Fine Mesh Agar With 400 Ml Of Distilled Water 4 Heat The Agar water Mixture In A Microwave Until Close To Boiling

With Fine Bubbles Throughout 5 Add 400 Ml Of Faucet temperature Distilled Water To The Agar Mixture To Lower The Temperature 6 Add The Agar Mixture To The Blender And Thoroughly Mix 7 Pour The Diet Mixture Into 4 ounce Diet Cups

Ensuring The Diet Covers The Bottom Of Each Cup 8 Allow The Diet To Cool

Then Place Lids On The Cups And Label Them With The Diet Type 9 Stack The Labeled Cups On Trays And House Them In A 24 C Climate Chamber

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