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Magnetic Separation and Negative Selection of Murine Neuronal Cultures

DOI :

10.3791/200391-v

•

2:43 min

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August 4th, 2023

August 4th, 2023

•
133 Views
1Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, Rutgers University

Tags

Magnetic Separation
Negative Selection
Murine Neuronal Cultures
Centrifugation
Brain Cell Suspension
HBSS With BSA Solution
Non neuronal Cell Biotin Antibody Cocktail
Anti biotin Microbeads
Cold Incubation
Column Rinsing
Cell Suspension Passing
Washes With HBSS BSA Solution
Magnetically Labeled Non neuronal Cells
Cell Centrifugation
Cell Resuspension
Neubauer Counting Chamber
Brightfield Microscope
LepR Positive Neurons
Axonal Extensions
Dendritic Processes
Immunofluorescent Studies

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