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Please note that all translations are automatically generated. Click here for the English version.
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01:52 min
July 7th, 2023
DOI :
10.3791/200419-v
Transcript
首先,使用生物反应器控制器对pH探头进行两点校准。然后,要组装生物反应器容器,请根据制造商的说明将不锈钢管和叶轮等部件安装到顶板上。按照制造商的指导,将即用型培养一次性耗材套件连接到头板上的相应不锈钢部件、管子和端口上。
组装容器后,按照制造商的说明对完全组装的容器进行气密性检查。用耗材套件在 15 PSI 和 121 摄氏度下高压灭菌容器 60 分钟。将容器冷却至室温后,将温度探头插入热电偶套管,并将监视器、pH 探头和 DO 探头的电缆从控制器连接到容器上的相应部件。
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