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Please note that all translations are automatically generated. Click here for the English version.
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01:50 min
October 6th, 2023
DOI :
10.3791/200454-v
* These authors contributed equally
Transcript
首先制备所需浓度的同源KPC肿瘤细胞的等分试样。将该细胞悬液储存在冰上的胰岛素注射器中。接下来,将麻醉的小鼠转移到无菌手术罩上,并将其置于部分右侧卧位。
用纸胶带固定四肢。用防腐剂对小鼠的腹部进行消毒。使用镊子和卡斯特罗维霍剪刀做一个 10 到 15 毫米长的左肋下皮肤切口。
必要时使用棉签控制止血。使用烧灼笔烧灼血管。接下来,将下面的肌肉分开进入腹膜。
用无菌棉签将胰腺和脾脏外化,以防止组织损伤。将胰腺张开以防止折叠。确定胰腺体或尾部的肿瘤注射点。</
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