Peking University People's Hospital

5 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Live Imaging of Innate Immune and Preneoplastic Cell Interactions Using an Inducible Gal4/UAS Expression System in Larval Zebrafish Skin

Thomas Ramezani¹, Derek W. Laux¹, Isabel R. Bravo¹, Masazumi Tada², Yi Feng¹

¹MRC Centre for Inflammation Research, Queen's Medical Research Institute, The University of Edinburgh, ²Department of Cell & Developmental Biology, University College London

Studying the earliest events of preneoplastic cell progression and innate immune cell interaction is pivotal to understand and treat cancer. Here we describe a method to conditionally induce epithelial cell transformations and the subsequent live imaging of innate immune cell interaction with HRAS^G12V expressing skin cells in zebrafish larvae.

Biology

Three-dimensional Reconstruction of the Vascular Architecture of the Passive CLARITY-cleared Mouse Ovary

Wei Hu^*1, Amin Tamadon^*1, Aaron J.W. Hsueh², Yi Feng¹

¹Department of Integrative Medicine and Neurobiology, School of Basic Medical Sciences; Institutes of Brain Science, Brain Science Collaborative Innovation Center, State Key Laboratory of Medical Neurobiology, Institute of Acupuncture and Moxibustion, Fudan Institutes of Integrative Medicine, Fudan University, ²Program of Reproductive and Stem Cell Biology, Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford University

Here we present an adaptation of the passive CLARITY and 3D reconstruction method for visualization of the ovarian vasculature and follicular capillaries in intact mouse ovaries.

Neuroscience

Microstate and Omega Complexity Analyses of the Resting-state Electroencephalography

Fei Gao¹, Huibin Jia², Yi Feng¹

¹Department of Pain Medicine, Peking University People's Hospital, ²Key Laboratory of Child Development and Learning Science of Ministry of Education, Research Center for Learning Science, School of Biological Sciences & Medical Engineering, Southeast University

This article describes the protocol underlying electroencephalography (EEG) microstate analysis and omega complexity analysis, which are two reference-free EEG measures and highly valuable to explore the neural mechanisms of brain disorders.

Medicine

Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma

Xu-yi Zhao¹, Yun-yue Yang¹, Jing-li Feng¹, Cui-ling Feng²

¹Dongzhimen Hospital affiliated to Beijing University of Chinese Medicine, ²Traditional Chinese Medicine Department, Peking University People's Hospital

This study reveals the mechanism of Trichosanthes-Fritillaria thunbergii in treating lung adenocarcinoma based on network pharmacology and experimental verification. The study also demonstrates that the PI3K/AKT signaling pathway plays a vital role in the action of Trichosanthes-Fritillaria thunbergii in treating lung adenocarcinoma.

Cancer Research

Stabilized Window for Intravital Imaging of the Murine Pancreas

Jakeb Petersen^*1,2,3, Wei Du^*4,5, Christian Adkisson^1,4, Claudia Gravekamp^6,7, Maja H. Oktay^2,3,4,7,8,9, John Condeelis^{1,3,4,7,8,9,10}, Nicole C. Panarelli^2,3,7, John C. McAuliffe^1,2,3,7,8, David Entenberg^2,3,4,7,8,9

We present a protocol for the surgical implantation of a stabilized indwelling optical window for subcellular-resolution imaging of the murine pancreas, allowing serial and longitudinal studies of the healthy and diseased pancreas.