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02:34 min
September 8th, 2023
DOI :
10.3791/200497-v
Transcript
首先,将准备好的脊髓切片放入记录室。然后将显微操作系统的阳极放置在阴极背中线附近,靠近背根进入区,以便正确定位脊髓刺激器。现在切换到 60 倍物镜,以识别具有光滑明亮表面而没有可见细胞核的健康神经元。
稍微降低红外强度,打开荧光光源,将滤光片调整为宽带紫外激发选项。现在,选择一个合适的氟金正运动神经元,并逐渐降低电极,直到它靠近神经元。当移液器接触其表面时,在吸头水平处寻找一个小的膜压痕,并释放先前施加的正压。
然后使用注射器对移液器施加负压,并等待软件界面上的电阻
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