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Please note that all translations are automatically generated. Click here for the English version.
837 Views
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03:05 min
May 5th, 2023
DOI :
10.3791/200498-v
Transcript
首先,在手术台上放置8至10周的麻醉C57黑色六雄性小鼠。使用钝镊子,拉出舌头并用手指握住。将喉镜插入口腔并观察声带。
在喉镜上稳定小鼠下巴,并握住20号静脉内或IV导管,其中插入导丝。然后将导丝轻轻插入声带,将 20 号静脉导管慢慢推入气管,直到其翼部与鼻尖齐平。将鼠标连接到呼吸机后,将其保持在横向位置,右侧颞区朝上。
使用加热垫和加热灯将直肠温度保持在 37 摄氏度。找到颧弓并切开颞肌后,用两把镊子解剖下面的颧弓,露出上颌骨和颧骨之间的关节。然后用剪刀剪下颧弓的
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