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DOI :
10.3791/2005-v
•
13:54 min
June 23rd, 2010
Chapters
0:00
Title
1:38
Preparing Apple Juice Agar Plates and Harvesting Embryos
3:26
Removing the Egg Shell From the Embryos (Dechorionation)
5:09
Mounting the Embroys in Agar
8:28
Centrifugation and Recovery of Embryos, Followed by Further Processing
13:00
Conclusion
0:43
Introduction
我们描述了独立的细胞器密度的方法,在生活果蝇胚胎。胚胎中嵌入琼脂,离心。这种技术产生沿前后胚胎轴重复性的主要细胞器的分离。这种方法有利于共定位实验和产量的细胞器组分生化分析和移植实验。
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