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Please note that all translations are automatically generated. Click here for the English version.
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02:14 min
August 11th, 2023
DOI :
10.3791/200508-v
Transcript
首先,使用切片机将福尔马林固定石蜡包埋的口腔粘膜下纤维化组织切成 10 微米厚度为 3 个的连续切片,然后在水中展开切片。将三个微米切片放在粘附显微镜载玻片或载玻片A上,将10微米切片放在笔膜载玻片或载玻片B上,将两个载玻片放在60摄氏度的热板上。两小时后,将载玻片 A 和 B 置于二甲苯中 10 分钟,然后在每种浓度下以分级乙醇浓度水合载玻片 1 分钟。
接下来,将载玻片放入超纯蒸馏水中 30 秒。将载玻片浸泡在Harris苏木精染料溶液中90秒。再次在超纯蒸馏水中洗涤载玻片
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