isolation-cells-with-morphological-spatial-information-from-oral

Isolation of Cells with Morphological and Spatial Information from Oral Submucous Fibrosis Samples by Laser Capture Microdissection

Oral submucous fibrosis (OSF) is a common type of potentially malignant disorder in the oral cavity. The atrophy of epithelium and fibrosis of the lamina ...

Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices

Chinese Academy of Medical Sciences (2019RU034)

Oral submucosal fibrosis was one of the oral potentially malignant disorders, which has become a global epidemic disease. This study aimed to investigate the clinicopathological features associated with the disease progression of oral submucosal fibrosis. We recruited 700 cases of oral submucosal fibrosis in the Department of oral pathology, Xiangya Stomatological Hospital, Central South University from July 1996 to July 2019, and analyzed the association among staging of oral submucosal fibrosis and age, sex, sites, duration of areca nut chewing. The age of the patients ranged from 14 to 63 years, with a median age of 32 years. The average age of oral submucosal fibrosis in the early stage (35.89 ± 9.97) was different from the average age in the middle stage (32.74 ± 8.83) and advanced stage (31.43 ± 7.57, P < 0.05). The risk of staging progression of oral submucosal fibrosis decreased with age (OR = 0.965, 95%CI: 0.945-0.986, P = 0.001).

Clinicopathological factors associated with progression of oral submucous fibrosis: A population-based retrospective study.

Oral lichen planus (OLP) was classified as an oral potentially malignant disorder due to the association with oral squamous cell carcinoma (OSCC). However, the malignant potential of OLP has been controversial. Whether epithelial dysplasia should be differentiated from OLP and lichenoid dysplasia could be identified as a pathological entity has been the subject of debate.

Overestimated risk of transformation in oral lichen planus.

To characterize the epidemiological, clinical, and prognostic features of multiple primary cancers (MPC) following oral squamous cell carcinoma (OSCC).

Clinical and prognostic features of multiple primary cancers with oral squamous cell carcinoma.

Head and neck squamous cell carcinoma (HNSCC) is a serious disease endangering the health of patients, and the application of immunotherapy in HNSCC is gradually emerging. However, there is no bibliometric analysis in this research field. This study aims to provide a comprehensive overview of the knowledge structure and research hotspots of immunotherapy for HNSCC.

Bibliometric analysis of immunotherapy for head and neck squamous cell carcinoma.

Oral leukoplakia concomitant with oral submucous fibrosis is a high-risk oral potentially malignant disorder, but little is known about its immune microenvironment.

The preliminary exploration of immune microenvironment in oral leukoplakia concomitant with oral submucosal fibrosis: A comparative immunohistochemical study.

Accurate prognostic stratification of oral leukoplakia (OLK) with risk of malignant transformation into oral squamous cell carcinoma is crucial. We developed an objective and powerful pathomics-based model for the prediction of malignant transformation in OLK using hematoxylin and eosin (H&E)-stained images. In total, 759 H&E-stained images from multicenter cohorts were included. A training set (n = 489), validation set (n = 196), and testing set (n = 74) were used for model development. Four deep learning methods were used to train and validate the model constructed using H&E-stained images. Pathomics features generated through deep learning combined with machine learning algorithms were used to develop a pathomics-based model. Immunohistochemical staining of Ki67, p53, and PD-L1 was used to interpret the black box of the model. Pathomics-based models predicted the malignant transformation of OLK (validation set area under curve [AUC], 0.899; testing set AUC, 0.813) and significantly identified high-risk and low-risk populations. The prediction performance of malignant transformation from dysplasia grading (validation set AUC, 0.743) was lower than that of the pathomics-based model. The expressions of Ki67, p53, and PD-L1 were correlated with various pathomics features. The pathomics-based model accurately predicted the malignant transformation of OLK and may be useful for the objective and rapid assessment of the prognosis of patients with OLK.

Development of a Pathomics-Based Model for the Prediction of Malignant Transformation in Oral Leukoplakia.

Oral leukoplakia is a common precursor lesion of oral squamous cell carcinoma, which indicates a high potential of malignancy. The malignant transformation of oral leukoplakia seriously affects patient survival and quality of life; however, it is difficult to identify oral leukoplakia patients who will develop carcinoma because no biomarker exists to predict malignant transformation for effective clinical management. As a major problem in the field of head and neck pathologies, it is imperative to identify biomarkers of malignant transformation in oral leukoplakia. In this review, we discuss the potential biomarkers of malignant transformation reported in the literature and explore the translational probabilities from bench to bedside. Although no single biomarker has yet been applied in the clinical setting, profiling for genomic instability might be a promising adjunct.

Article	Year
Clinicopathological factors associated with progression of oral submucous fibrosis: A population-based retrospective study. Oral oncology\| PubMed ID: 35662030	2022
Overestimated risk of transformation in oral lichen planus. Oral oncology\| PubMed ID: 35858493	2022
Clinical and prognostic features of multiple primary cancers with oral squamous cell carcinoma. Archives of oral biology\| PubMed ID: 36857878	2023
Bibliometric analysis of immunotherapy for head and neck squamous cell carcinoma. Journal of dental sciences\| PubMed ID: 37021217	2023
The preliminary exploration of immune microenvironment in oral leukoplakia concomitant with oral submucosal fibrosis: A comparative immunohistochemical study. Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology\| PubMed ID: 37084341	2023
Development of a Pathomics-Based Model for the Prediction of Malignant Transformation in Oral Leukoplakia. Laboratory investigation; a journal of technical methods and pathology\| PubMed ID: 37164265	2023
Biomarkers of malignant transformation in oral leukoplakia: from bench to bedside. Journal of Zhejiang University. Science. B\| PubMed ID: 37183571	2023

Xinjia Cai

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