Name: Single-Leg Knee-Extensor Chair Setup and Ultrasound Apparatus Configuration for Leg Blood Flow Assessment
Uploaded: 2023-12-15T13:50:00
Description: Watch this Scientific Journal Video about Single-Leg Knee-Extensor Chair Setup and Ultrasound Apparatus Configuration for Leg Blood Flow Assessment at JoVE.com

single-leg knee-extensor chair setup and ultrasound apparatus configuration for leg blood flow assessment

Bedömning av blodflödet i benen vid träningsbelastning

Doppler ultrasound, introduced in the 1980s, has been extensively used to determine contracting muscle blood flow, particularly in the single-leg knee-extensor model, allowing measurement of blood flow in the common femoral artery (CFA) during small muscle mass activation1,2,3,4,5,6. Doppler ultrasound-based blood flow technology has provided valuable insights into vascular regulation in various populations, including healthy adults7,8, individuals with diabetes9, hypertension10, COPD11,12, and heart failure13,14.
One advantage of Doppler ultrasound is its non-invasiveness compared to other blood flow determination methods like thermodilution, and it can be combined with arterial and venous catheterization if necessary3,4,6,15. It also enables beat-to-beat blood flow velocity measurement, allowing for the detection of rapid changes16. However, Doppler ultrasound-based blood measurements have limitations, including difficulties in obtaining stable recordings during excessive limb movement at near-maximal exercise intensities and the requirement for ultrasound accessibility to the targeted blood vessel, excluding evaluations during ergometer bicycling15. Hence, the single-leg knee-extensor model is well-suited for LBF evaluation using Doppler ultrasound during dynamic exercise at submaximal intensities17, minimizing the influence of exercise-related heart and lung limitations and facilitating comparisons between healthy subjects and patients with cardio-pulmonary diseases11.
Despite being widely used, the between-day reliability of the single-leg knee-extensor model using Doppler ultrasound has not been investigated on a larger scale in recent decades, with prior studies involving small populations (n = 2)3,18,19,20.
This study aimed to investigate (1) the within-day test-retest reliability, (2) the between-day test-retest reliability, and (3) the inter-rater reliability of Doppler ultrasound for LBF evaluation during single-leg knee-extensor exercise at 0 W, 6 W, 12 W, and 18 W. The measurements were conducted in a clinically realistic scenario where the probe was removed between measurements. It is important to note that several intrinsic and extrinsic environmental factors known to influence LBF were not controlled during the measurements, which could introduce variability and affect reliability. Considering advancements in Doppler ultrasound technology and blood flow analysis software, we hypothesized that, even in an uncontrolled setting, acceptable within- and between-day reliability of LBF measurements could be achieved at all intensities when performed by the same sonographer.

Författare i fokus: Bedömning av tillförlitligheten hos dopplerultraljud vid mätning av benblodflöde 

Doppler ultraljudsbaserad benblodflödesbedömning under enbens knästräckarövning i en okontrollerad miljö

This method is widely recognized and applied in numerous clinical and research settings. The primary objective of this study was to assist the reproducibility of the method, thereby providing valuable insights into the measurement error. By doing so, we can say what is genuine physiological change and what is due to measurement error.Ultimately, this will enhance our understanding of this method. The primary obstacle lies in exploring physiological changes during various activities in everyday life, as conducting such investigation is feasible only under specific experimental setups. t has been over two decades since the last reliability study was conducted during single leg knee extensor exercise.Over this period, technology has significantly evolved, making it imperative to revisit this research area to gain valuable insight into the measurement era associated with the current method. Doppler ultrasound is non-invasive compared to other blood flow determination methods like Thermodilution, and it can be combined with arterial and venous catheterization if necessary. It also enables beat to beat blood flow velocity measurements so we could detect rapid changes.In the future, our main goal is to investigate the regulation of blood flow to contracting skeletal muscle in patients with chronic obstructive pulmonary disease.

Watch this Scientific Journal Video about Single-Leg Knee-Extensor Chair Setup and Ultrasound Apparatus Configuration for Leg Blood Flow Assessment at JoVE.com

Single-Leg Knee-Extensor Chair Setup and Ultrasound Apparatus Configuration for Leg Blood Flow Assessment  

Enbens knästräckarstol och ultraljudsapparatkonfiguration för benblodflödesbedömning

Till att börja med, placera deltagaren i enbensknästräckarstolen med ryggen vilande mot stolen. Placera en stol eller bänk för att stabilisera det inaktiva benet. För att säkerställa att elektroderna är på samma avstånd från hjärtat, fäst en EKG-elektrod på höger sida av bröstväggen i det tredje interkostala utrymmet.Placera den andra på vänster sida i det tredje interkostala utrymmet. Placera sedan den tredje på vänster sida i det 11:e interkostala utrymmet. Efter elektrodplacering, placera deltagaren i en vinkel som är större än 90 grader mellan buken och låret.Justera armen som ansluter den enkla knästräckarstolen till svänghjulet. Detta gör det möjligt för deltagaren att sträcka ut knäet helt. Knyt benet hårt till stolens pedal för att undvika användning av muskler i den nedre delen av lemmen.Tryck sedan på påslagningsknappen på ultraljudsapparaten. Tryck på patienten för att skapa en ny fil för undersökningen. Flytta markören till ny patient och tryck på enter.Fyll sedan i patient-ID.Flytta markören för att skapa och tryck på enter. Anslut ledningen till elektroderna. Tryck på sonden och välj den linjära sonden.Applicera ultraljudsgel på sonden.