Name: Analyzing Micro-Computed Tomography (CT) Images of Murine Trabecular Bone
Uploaded: 2023-09-08T14:10:00
Description: Watch this Scientific Journal Video about Analyzing Micro-Computed Tomography CT Images of Murine Trabecular Bone at JoVE.com

analyzing micro-computed tomography (ct) images of murine trabecular bone

HE-farvning og mikro-CT til evaluering af murin trabekulær knoglemikroarkitektur

Osteoporosis is a prevalent metabolic bone disease, especially among the elderly, and is associated with an increased risk of fragility fractures. As osteoporosis becomes more common in China1, there will be a growing demand for studying the bone structures of small animals2,3. The previous methods of measuring bone loss rely on the outcomes of two-dimensional dual-energy x-ray absorptiometry. However, this does not capture the changes in the architectural microstructure of the trabecular bone, which is a key factor for skeletal strength4. The microstructure of bone affects its strength, stiffness, and fracture resistance. By comparing bone microarchitecture in normal and pathological states, changes in bone tissue morphology, structure, and function caused by osteoporosis can be identified. This information contributes to the understanding of the development of osteoporosis and its association with other diseases. 
Micro-computed tomography (Micro-CT) imaging has recently become a popular technique for bone morphology assessment, where it can provide accurate and comprehensive data on bone structure and density parameters such as bone volume fraction, thickness, and separation5,6. At the same time, the Micro-CT results can be affected by the analysis software7. Different methods of image acquisition, evaluation, and reporting are used by various commercial Micro-CT systems. This inconsistency makes it hard to compare and interpret the results reported by different studies5. Also, it cannot currently replace bone histomorphometry in providing researchers with information on cellular-level parameters in the skeletal system8. Meanwhile, histologic techniques allow direct observation and measurement of the microscopic morphology of bone. Hematoxylin and eosin (HE) staining is a common staining technique used in histology to visualize the general structure of cells and tissues. It is used to identify the presence of bone tissue and its microarchitecture.
This article uses Micro-CT combined with tissue slicing technique (Hematoxylin-Eosin [HE] staining) to collect bone tissue images and perform quantitative analysis of trabecular bone to evaluate the changes of bone microstructure in an osteoporosis mouse model.

Forfatter Spotlight: En økonomisk og effektiv metode til kvantitativ evaluering af knoglemikroarkitektur i en murine osteoporosemodel 

Trabekulær knoglemikroarkitektur evaluering i en osteoporose mus model

Our research focused on the effects of traditional Chinese medicine, or TCM, on osteoporosis. We are trying to explore the mechanism, efficacy, and safety of TCM intervention for osteoporosis. Micro-computed tomography imaging has recently become a popular technique for bone morphology assessment.It can provide accurate and comprehensive data on bone structure and density parameters, such as bone volume, fracture thickness, and separation. The skeletal system is the most unique tissue structure in terms of the physical properties of the body. It contains the inorganic component arginine calcium phosphate, and it is hard and stiff as a result.The process of hurting bone symptoms is tedious. We provided a complete and detailed process from modeling to osteoporosis in evaluation for researchers. We believe the application of the technology will undoubtedly bring great improvements to osteoporosis research in the context of ethnomedicine exploration.

Watch this Scientific Journal Video about Analyzing Micro-Computed Tomography CT Images of Murine Trabecular Bone at JoVE.com

Analyzing Micro-Computed Tomography (CT) Images of Murine Trabecular Bone  

Analyse af mikrocomputertomografi (CT) billeder af murin trabekulær knogle

For at begynde at analysere mikrocomputertomografi eller mikro-CT-billeder i mikro-CT-billedbehandlingssystemsoftwaren skal du vælge de data, der skal analyseres. Klik på Sub, og indstil pixelstørrelsen til 10 mikron. Flyt og tilpas størrelsen på interesseområdet, eller ROI, for at inkludere det distale lårben over vækstpladen.Klik derefter på Start. Klik derefter på Analyse 3D knappen for at få den resulterende 3D-rekonstruktion. Eksporter dataene fra mikro-CT-systemet til computeren til analyse.Når du har importeret de rekonstruerede CT-data, skal du klikke på Proces- og billedberegner. Klik derefter sekventielt på Region Pad og Interaktiv. Juster det gule afkrydsningsfelt til det relevante investeringsafkast.Når du har afsluttet billedberegneren, skal du vælge knoglemikroarkitekturanalysen eller BMA-tilføjelsen og klikke på Segmenter Cortex og Segment Trabeculae. Klik til sidst på Mål knogle for at beregne knoglemorfometriske indekser. De trabekulære mikroarkitektoniske parametre blev målt i mus fra to grupper, den skamopererede gruppe og den ovariektomiserede osteoporosemodelgruppe eller OVX-gruppen.Resultaterne viste signifikante forskelle mellem mus fra de to grupper. Forholdet mellem knoglevolumen og totalt vævsvolumen i OVX-gruppen var 3% lavere end i skingruppen. Den trabekulære tykkelse hos mus fra OVX-gruppen var lavere end i skingruppen, og den trabekulære adskillelse i OVX-musene var større end hos skinmusene.3D-visninger af trabekulære ROI'er ekstraheret fra rekonstrueret knoglevolumen afslørede, at sammenlignet med sham-gruppen i OVX-musene var trabeculae sparsom og viste osteoporose.

Research

JoVE Journal

Medicine

Analyzing Micro-Computed Tomography (CT) Images of Murine Trabecular Bone

Trabecular Bone Microarchitecture Evaluation in an Osteoporosis Mouse Model

Bone microstructure refers to the arrangement and quality of bone tissue at the microscopic level. Understanding the bone microstructure of the skeleton ...