Name: Analyzing Micro-Computed Tomography (CT) Images of Murine Trabecular Bone
Uploaded: 2023-09-08T14:10:00
Description: Watch this Scientific Journal Video about Analyzing Micro-Computed Tomography CT Images of Murine Trabecular Bone at JoVE.com

analyzing micro-computed tomography (ct) images of murine trabecular bone

HE-kleuring en micro-CT om de microarchitectuur van het trabeculaire bot van muizen te evalueren

Osteoporosis is a prevalent metabolic bone disease, especially among the elderly, and is associated with an increased risk of fragility fractures. As osteoporosis becomes more common in China1, there will be a growing demand for studying the bone structures of small animals2,3. The previous methods of measuring bone loss rely on the outcomes of two-dimensional dual-energy x-ray absorptiometry. However, this does not capture the changes in the architectural microstructure of the trabecular bone, which is a key factor for skeletal strength4. The microstructure of bone affects its strength, stiffness, and fracture resistance. By comparing bone microarchitecture in normal and pathological states, changes in bone tissue morphology, structure, and function caused by osteoporosis can be identified. This information contributes to the understanding of the development of osteoporosis and its association with other diseases. 
Micro-computed tomography (Micro-CT) imaging has recently become a popular technique for bone morphology assessment, where it can provide accurate and comprehensive data on bone structure and density parameters such as bone volume fraction, thickness, and separation5,6. At the same time, the Micro-CT results can be affected by the analysis software7. Different methods of image acquisition, evaluation, and reporting are used by various commercial Micro-CT systems. This inconsistency makes it hard to compare and interpret the results reported by different studies5. Also, it cannot currently replace bone histomorphometry in providing researchers with information on cellular-level parameters in the skeletal system8. Meanwhile, histologic techniques allow direct observation and measurement of the microscopic morphology of bone. Hematoxylin and eosin (HE) staining is a common staining technique used in histology to visualize the general structure of cells and tissues. It is used to identify the presence of bone tissue and its microarchitecture.
This article uses Micro-CT combined with tissue slicing technique (Hematoxylin-Eosin [HE] staining) to collect bone tissue images and perform quantitative analysis of trabecular bone to evaluate the changes of bone microstructure in an osteoporosis mouse model.

Auteur in de schijnwerpers: een economische en efficiënte methode voor kwantitatieve evaluatie van botmicroarchitectuur in een muizen osteoporosemodel 

Evaluatie van de microarchitectuur van het trabeculaire bot in een muismodel met osteoporose

Our research focused on the effects of traditional Chinese medicine, or TCM, on osteoporosis. We are trying to explore the mechanism, efficacy, and safety of TCM intervention for osteoporosis. Micro-computed tomography imaging has recently become a popular technique for bone morphology assessment.It can provide accurate and comprehensive data on bone structure and density parameters, such as bone volume, fracture thickness, and separation. The skeletal system is the most unique tissue structure in terms of the physical properties of the body. It contains the inorganic component arginine calcium phosphate, and it is hard and stiff as a result.The process of hurting bone symptoms is tedious. We provided a complete and detailed process from modeling to osteoporosis in evaluation for researchers. We believe the application of the technology will undoubtedly bring great improvements to osteoporosis research in the context of ethnomedicine exploration.

Watch this Scientific Journal Video about Analyzing Micro-Computed Tomography CT Images of Murine Trabecular Bone at JoVE.com

Analyzing Micro-Computed Tomography (CT) Images of Murine Trabecular Bone  

Analyse van microcomputertomografie (CT)-beelden van trabeculair bot bij muizen

Om te beginnen met het analyseren van de microcomputertomografie, of micro-CT-beelden, in de software van het micro-CT-beeldvormingssysteem, selecteert u de gegevens die moeten worden geanalyseerd. Klik op Sub en stel de pixelgrootte in op 10 micron. Verplaats en wijzig de grootte van het interessegebied, of ROI, om het distale dijbeen boven de groeischijf op te nemen.Klik vervolgens op Start. Klik vervolgens op de Analyse 3D knop om de resulterende 3D-reconstructie te krijgen. Exporteer de gegevens van het micro-CT-systeem naar de computer voor analyse.Klik na het importeren van de gereconstrueerde CT-gegevens op Proces- en afbeeldingscalculator. Klik vervolgens achtereenvolgens op Regiopad en Interactief. Pas het gele selectievakje aan op de juiste ROI.Nadat u de afbeeldingscalculator hebt afgesloten, selecteert u de botmicroarchitectuuranalyse of BMA-add-on en klikt u op Segmentcortex en Segmenttrabeculae. Klik ten slotte op Bot meten om de botmorfometrische indices te berekenen. De trabeculaire micro-architecturale parameters werden gemeten bij muizen uit twee groepen, de schijnoperatiegroep en de ovariëctomische osteoporosemodelgroep of OVX-groep.De resultaten wezen op significante verschillen tussen muizen van de twee groepen. De verhouding tussen botvolume en totaal weefselvolume in de OVX-groep was 3% lager dan die in de schijngroep. De trabeculaire dikte bij muizen van de OVX-groep was lager dan bij de schijngroep, en de trabeculaire scheiding bij de OVX-muizen was groter dan die bij de schijnmuizen.3D-weergaven van trabeculaire ROI's geëxtraheerd uit gereconstrueerd botvolume onthulden dat in vergelijking met de schijngroep, in de OVX-muizen, de trabeculae schaars waren en osteoporose vertoonden.

Research

JoVE Journal

Medicine

Analyzing Micro-Computed Tomography (CT) Images of Murine Trabecular Bone

Trabecular Bone Microarchitecture Evaluation in an Osteoporosis Mouse Model

Bone microstructure refers to the arrangement and quality of bone tissue at the microscopic level. Understanding the bone microstructure of the skeleton ...