Name: Analyzing Micro-Computed Tomography (CT) Images of Murine Trabecular Bone
Uploaded: 2023-09-08T14:10:00
Description: Watch this Scientific Journal Video about Analyzing Micro-Computed Tomography CT Images of Murine Trabecular Bone at JoVE.com

analyzing micro-computed tomography (ct) images of murine trabecular bone

マウス海綿骨の微細構造を評価するためのHE染色とマイクロCT

Osteoporosis is a prevalent metabolic bone disease, especially among the elderly, and is associated with an increased risk of fragility fractures. As osteoporosis becomes more common in China1, there will be a growing demand for studying the bone structures of small animals2,3. The previous methods of measuring bone loss rely on the outcomes of two-dimensional dual-energy x-ray absorptiometry. However, this does not capture the changes in the architectural microstructure of the trabecular bone, which is a key factor for skeletal strength4. The microstructure of bone affects its strength, stiffness, and fracture resistance. By comparing bone microarchitecture in normal and pathological states, changes in bone tissue morphology, structure, and function caused by osteoporosis can be identified. This information contributes to the understanding of the development of osteoporosis and its association with other diseases. 
Micro-computed tomography (Micro-CT) imaging has recently become a popular technique for bone morphology assessment, where it can provide accurate and comprehensive data on bone structure and density parameters such as bone volume fraction, thickness, and separation5,6. At the same time, the Micro-CT results can be affected by the analysis software7. Different methods of image acquisition, evaluation, and reporting are used by various commercial Micro-CT systems. This inconsistency makes it hard to compare and interpret the results reported by different studies5. Also, it cannot currently replace bone histomorphometry in providing researchers with information on cellular-level parameters in the skeletal system8. Meanwhile, histologic techniques allow direct observation and measurement of the microscopic morphology of bone. Hematoxylin and eosin (HE) staining is a common staining technique used in histology to visualize the general structure of cells and tissues. It is used to identify the presence of bone tissue and its microarchitecture.
This article uses Micro-CT combined with tissue slicing technique (Hematoxylin-Eosin [HE] staining) to collect bone tissue images and perform quantitative analysis of trabecular bone to evaluate the changes of bone microstructure in an osteoporosis mouse model.

著者スポットライト:マウス骨粗鬆症モデルにおける骨マイクロアーキテクチャの定量的評価のための経済的かつ効率的な方法 

骨粗鬆症マウスモデルにおける海綿骨マイクロアーキテクチャ評価

Our research focused on the effects of traditional Chinese medicine, or TCM, on osteoporosis. We are trying to explore the mechanism, efficacy, and safety of TCM intervention for osteoporosis. Micro-computed tomography imaging has recently become a popular technique for bone morphology assessment.It can provide accurate and comprehensive data on bone structure and density parameters, such as bone volume, fracture thickness, and separation. The skeletal system is the most unique tissue structure in terms of the physical properties of the body. It contains the inorganic component arginine calcium phosphate, and it is hard and stiff as a result.The process of hurting bone symptoms is tedious. We provided a complete and detailed process from modeling to osteoporosis in evaluation for researchers. We believe the application of the technology will undoubtedly bring great improvements to osteoporosis research in the context of ethnomedicine exploration.

Watch this Scientific Journal Video about Analyzing Micro-Computed Tomography CT Images of Murine Trabecular Bone at JoVE.com

Analyzing Micro-Computed Tomography (CT) Images of Murine Trabecular Bone  

マウス海綿骨のマイクロコンピュータ断層撮影(CT)画像の解析

マイクロコンピュータ断層撮影(マイクロCT画像)の解析を開始するには、マイクロCTイメージングシステムソフトウェアで、解析するデータを選択します。[サブ] をクリックし、ピクセル サイズを 10 ミクロンに設定します。関心領域(ROI)を移動してサイズを変更し、成長プレートの上の大腿骨遠位部を含めます。次に、[開始]をクリックします。次に、[解析 3D] ボタンをクリックして、結果の 3D 再構成を取得します。マイクロCTシステムからコンピューターにデータをエクスポートして分析します。再構成されたCTデータをインポートした後、Process and Image Calculatorをクリックします。次に、「Region Pad」と「Interactive」を順番にクリックします。黄色のチェックボックスを適切なROIに調整します。画像計算機を終了した後、骨マイクロアーキテクチャ解析またはBMAアドオンを選択し、Segment CortexとSegment Trabeculaeをクリックします。最後に、Measure Boneをクリックして、骨の形態指数を計算します。小柱の微細構造パラメータは、偽手術群と卵巣摘出骨粗鬆症モデル群(OVX群)の2つのグループのマウスで測定されました。その結果、2群のマウス間で有意差が認められました。OVX群の総組織体積に対する骨体積の比率は、偽群よりも3%低かった。OVX群のマウスの小柱の厚さは偽群よりも低く、OVXマウスの小柱の分離は偽マウスよりも大きかった。再建された骨量から抽出した小柱ROIの3D表示により、OVXマウスでは偽群と比較して、小柱がまばらで骨粗鬆症を示すことが明らかになりました。

Research

JoVE Journal

Medicine

Analyzing Micro-Computed Tomography (CT) Images of Murine Trabecular Bone

Trabecular Bone Microarchitecture Evaluation in an Osteoporosis Mouse Model

Bone microstructure refers to the arrangement and quality of bone tissue at the microscopic level. Understanding the bone microstructure of the skeleton ...