Name: Analyzing Micro-Computed Tomography (CT) Images of Murine Trabecular Bone
Uploaded: 2023-09-08T14:10:00
Description: Watch this Scientific Journal Video about Analyzing Micro-Computed Tomography CT Images of Murine Trabecular Bone at JoVE.com

analyzing micro-computed tomography (ct) images of murine trabecular bone

쥐 섬유주 뼈 미세건축을 평가하기 위한 HE 염색 및 Micro-CT

Osteoporosis is a prevalent metabolic bone disease, especially among the elderly, and is associated with an increased risk of fragility fractures. As osteoporosis becomes more common in China1, there will be a growing demand for studying the bone structures of small animals2,3. The previous methods of measuring bone loss rely on the outcomes of two-dimensional dual-energy x-ray absorptiometry. However, this does not capture the changes in the architectural microstructure of the trabecular bone, which is a key factor for skeletal strength4. The microstructure of bone affects its strength, stiffness, and fracture resistance. By comparing bone microarchitecture in normal and pathological states, changes in bone tissue morphology, structure, and function caused by osteoporosis can be identified. This information contributes to the understanding of the development of osteoporosis and its association with other diseases. 
Micro-computed tomography (Micro-CT) imaging has recently become a popular technique for bone morphology assessment, where it can provide accurate and comprehensive data on bone structure and density parameters such as bone volume fraction, thickness, and separation5,6. At the same time, the Micro-CT results can be affected by the analysis software7. Different methods of image acquisition, evaluation, and reporting are used by various commercial Micro-CT systems. This inconsistency makes it hard to compare and interpret the results reported by different studies5. Also, it cannot currently replace bone histomorphometry in providing researchers with information on cellular-level parameters in the skeletal system8. Meanwhile, histologic techniques allow direct observation and measurement of the microscopic morphology of bone. Hematoxylin and eosin (HE) staining is a common staining technique used in histology to visualize the general structure of cells and tissues. It is used to identify the presence of bone tissue and its microarchitecture.
This article uses Micro-CT combined with tissue slicing technique (Hematoxylin-Eosin [HE] staining) to collect bone tissue images and perform quantitative analysis of trabecular bone to evaluate the changes of bone microstructure in an osteoporosis mouse model.

저자 스포트라이트: 쥐 골다공증 모델에서 뼈 미세구조의 정량적 평가를 위한 경제적이고 효율적인 방법 

골다공증 마우스 모델에서의 섬유주 뼈 미세구조 평가

Our research focused on the effects of traditional Chinese medicine, or TCM, on osteoporosis. We are trying to explore the mechanism, efficacy, and safety of TCM intervention for osteoporosis. Micro-computed tomography imaging has recently become a popular technique for bone morphology assessment.It can provide accurate and comprehensive data on bone structure and density parameters, such as bone volume, fracture thickness, and separation. The skeletal system is the most unique tissue structure in terms of the physical properties of the body. It contains the inorganic component arginine calcium phosphate, and it is hard and stiff as a result.The process of hurting bone symptoms is tedious. We provided a complete and detailed process from modeling to osteoporosis in evaluation for researchers. We believe the application of the technology will undoubtedly bring great improvements to osteoporosis research in the context of ethnomedicine exploration.

Watch this Scientific Journal Video about Analyzing Micro-Computed Tomography CT Images of Murine Trabecular Bone at JoVE.com

Analyzing Micro-Computed Tomography (CT) Images of Murine Trabecular Bone  

쥐 섬유주 뼈의 마이크로 컴퓨터 단층 촬영(CT) 이미지 분석

마이크로 CT 이미징 시스템 소프트웨어에서 마이크로 컴퓨터 단층 촬영 또는 마이크로 CT 이미지 분석을 시작하려면 분석할 데이터를 선택합니다. [하위]를 클릭하고 픽셀 크기를 10미크론으로 설정합니다. 관심 영역(ROI)을 이동하고 크기를 조정하여 성장판 위의 대퇴골 원위부를 포함합니다.그런 다음 시작을 클릭합니다. 그런 다음 3D 분석 버튼을 클릭하여 결과 3D 재구성을 가져옵니다. 분석을 위해 마이크로 CT 시스템에서 컴퓨터로 데이터를 내보냅니다.복원된 CT 데이터를 가져온 후 프로세스 및 영상 계산기(Process and Image Calculator)를 클릭합니다. 그런 다음 Region Pad 및 Interactive를 순차적으로 클릭합니다. 노란색 체크박스를 적절한 ROI로 조정합니다.이미지 계산기를 종료한 후 뼈 미세아키텍처 분석 또는 BMA 애드온을 선택하고 Segment Cortex and Segment Trabeculae를 클릭합니다. 마지막으로 본 측정(Measure Bone) 을 클릭하여 본 형태 지수를 계산합니다. 섬유주 미세구조 매개변수는 가짜 수술군과 난소절제술 골다공증 모델군(OVX group)의 두 그룹의 마우스에서 측정되었습니다.그 결과 두 그룹의 쥐 간에 유의미한 차이가 나타났다. OVX 그룹의 전체 조직 부피에 대한 뼈 부피의 비율은 가짜 그룹보다 3% 낮았습니다. OVX 그룹의 마우스에서의 섬유주 두께는 가짜 마우스보다 낮았고, OVX 마우스의 섬유주 분리는 가짜 마우스보다 컸다.재건된 뼈 용적에서 추출한 섬유주 ROI의 3D 디스플레이는 가짜 그룹에 비해 OVX 마우스에서 섬유주가 희박하고 골다공증을 보였다는 것을 보여주었습니다.

Research

JoVE Journal

Medicine

Analyzing Micro-Computed Tomography (CT) Images of Murine Trabecular Bone

Trabecular Bone Microarchitecture Evaluation in an Osteoporosis Mouse Model

Bone microstructure refers to the arrangement and quality of bone tissue at the microscopic level. Understanding the bone microstructure of the skeleton ...