Name: Analyzing Micro-Computed Tomography (CT) Images of Murine Trabecular Bone
Uploaded: 2023-09-08T14:10:00
Description: Watch this Scientific Journal Video about Analyzing Micro-Computed Tomography CT Images of Murine Trabecular Bone at JoVE.com

analyzing micro-computed tomography (ct) images of murine trabecular bone

Tinción de HE y micro-TC para evaluar la microarquitectura del hueso trabecular murino

Osteoporosis is a prevalent metabolic bone disease, especially among the elderly, and is associated with an increased risk of fragility fractures. As osteoporosis becomes more common in China1, there will be a growing demand for studying the bone structures of small animals2,3. The previous methods of measuring bone loss rely on the outcomes of two-dimensional dual-energy x-ray absorptiometry. However, this does not capture the changes in the architectural microstructure of the trabecular bone, which is a key factor for skeletal strength4. The microstructure of bone affects its strength, stiffness, and fracture resistance. By comparing bone microarchitecture in normal and pathological states, changes in bone tissue morphology, structure, and function caused by osteoporosis can be identified. This information contributes to the understanding of the development of osteoporosis and its association with other diseases. 
Micro-computed tomography (Micro-CT) imaging has recently become a popular technique for bone morphology assessment, where it can provide accurate and comprehensive data on bone structure and density parameters such as bone volume fraction, thickness, and separation5,6. At the same time, the Micro-CT results can be affected by the analysis software7. Different methods of image acquisition, evaluation, and reporting are used by various commercial Micro-CT systems. This inconsistency makes it hard to compare and interpret the results reported by different studies5. Also, it cannot currently replace bone histomorphometry in providing researchers with information on cellular-level parameters in the skeletal system8. Meanwhile, histologic techniques allow direct observation and measurement of the microscopic morphology of bone. Hematoxylin and eosin (HE) staining is a common staining technique used in histology to visualize the general structure of cells and tissues. It is used to identify the presence of bone tissue and its microarchitecture.
This article uses Micro-CT combined with tissue slicing technique (Hematoxylin-Eosin [HE] staining) to collect bone tissue images and perform quantitative analysis of trabecular bone to evaluate the changes of bone microstructure in an osteoporosis mouse model.

Autor destacado: Un método económico y eficiente para la evaluación cuantitativa de la microarquitectura ósea en un modelo murino de osteoporosis 

Evaluación de la microarquitectura ósea trabecular en un modelo murino de osteoporosis

Our research focused on the effects of traditional Chinese medicine, or TCM, on osteoporosis. We are trying to explore the mechanism, efficacy, and safety of TCM intervention for osteoporosis. Micro-computed tomography imaging has recently become a popular technique for bone morphology assessment.It can provide accurate and comprehensive data on bone structure and density parameters, such as bone volume, fracture thickness, and separation. The skeletal system is the most unique tissue structure in terms of the physical properties of the body. It contains the inorganic component arginine calcium phosphate, and it is hard and stiff as a result.The process of hurting bone symptoms is tedious. We provided a complete and detailed process from modeling to osteoporosis in evaluation for researchers. We believe the application of the technology will undoubtedly bring great improvements to osteoporosis research in the context of ethnomedicine exploration.

Watch this Scientific Journal Video about Analyzing Micro-Computed Tomography CT Images of Murine Trabecular Bone at JoVE.com

Analyzing Micro-Computed Tomography (CT) Images of Murine Trabecular Bone  

Análisis de imágenes de microtomografía computarizada (TC) del hueso trabecular murino

Para comenzar a analizar las imágenes de microtomografía computarizada, o micro CT, en el software del sistema de imágenes de micro CT, seleccione los datos que se van a analizar. Haga clic en Sub y establezca el tamaño de píxel en 10 micras. Mueva y cambie el tamaño de la región de interés, o ROI, para incluir el fémur distal por encima del cartílago de crecimiento.A continuación, haz clic en Iniciar. A continuación, haga clic en el botón Análisis 3D para obtener la reconstrucción 3D resultante. Exporte los datos del sistema de micro TC a la computadora para su análisis.Después de importar los datos de TC reconstruidos, haga clic en Calculadora de proceso e imagen. A continuación, haga clic secuencialmente en Pad de pasaje e Interactivo. Ajuste la casilla de verificación amarilla al ROI adecuado.Después de salir de la calculadora de imágenes, seleccione el complemento de análisis de microarquitectura ósea o BMA y haga clic en Segmentar corteza y Segmentar trabéculas. Por último, haz clic en Medir hueso para calcular los índices morfométricos óseos. Los parámetros microarquitectónicos trabeculares se midieron en ratones de dos grupos, el grupo operado simuladamente y el grupo modelo de osteoporosis ovariectomizada, o grupo OVX.Los resultados indicaron diferencias significativas entre los ratones de los dos grupos. La relación entre el volumen óseo y el volumen total de tejido en el grupo OVX fue un 3% menor que en el grupo simulado. El grosor trabecular en los ratones del grupo OVX fue menor que en el grupo simulado, y la separación trabecular en los ratones OVX fue mayor que en los ratones simulados.Las representaciones en 3D de las ROI trabeculares extraídas del volumen óseo reconstruido revelaron que, en comparación con el grupo simulado, en los ratones OVX, las trabéculas eran escasas y mostraban osteoporosis.

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Medicine

Analyzing Micro-Computed Tomography (CT) Images of Murine Trabecular Bone

Trabecular Bone Microarchitecture Evaluation in an Osteoporosis Mouse Model

Bone microstructure refers to the arrangement and quality of bone tissue at the microscopic level. Understanding the bone microstructure of the skeleton ...