Name: Analyzing Micro-Computed Tomography (CT) Images of Murine Trabecular Bone
Uploaded: 2023-09-08T14:10:00
Description: Watch this Scientific Journal Video about Analyzing Micro-Computed Tomography CT Images of Murine Trabecular Bone at JoVE.com

analyzing micro-computed tomography (ct) images of murine trabecular bone

HE-färgning och mikro-CT för att utvärdera murina trabekulära benmikroarkitektur

Osteoporosis is a prevalent metabolic bone disease, especially among the elderly, and is associated with an increased risk of fragility fractures. As osteoporosis becomes more common in China1, there will be a growing demand for studying the bone structures of small animals2,3. The previous methods of measuring bone loss rely on the outcomes of two-dimensional dual-energy x-ray absorptiometry. However, this does not capture the changes in the architectural microstructure of the trabecular bone, which is a key factor for skeletal strength4. The microstructure of bone affects its strength, stiffness, and fracture resistance. By comparing bone microarchitecture in normal and pathological states, changes in bone tissue morphology, structure, and function caused by osteoporosis can be identified. This information contributes to the understanding of the development of osteoporosis and its association with other diseases. 
Micro-computed tomography (Micro-CT) imaging has recently become a popular technique for bone morphology assessment, where it can provide accurate and comprehensive data on bone structure and density parameters such as bone volume fraction, thickness, and separation5,6. At the same time, the Micro-CT results can be affected by the analysis software7. Different methods of image acquisition, evaluation, and reporting are used by various commercial Micro-CT systems. This inconsistency makes it hard to compare and interpret the results reported by different studies5. Also, it cannot currently replace bone histomorphometry in providing researchers with information on cellular-level parameters in the skeletal system8. Meanwhile, histologic techniques allow direct observation and measurement of the microscopic morphology of bone. Hematoxylin and eosin (HE) staining is a common staining technique used in histology to visualize the general structure of cells and tissues. It is used to identify the presence of bone tissue and its microarchitecture.
This article uses Micro-CT combined with tissue slicing technique (Hematoxylin-Eosin [HE] staining) to collect bone tissue images and perform quantitative analysis of trabecular bone to evaluate the changes of bone microstructure in an osteoporosis mouse model.

Författare Spotlight: En ekonomisk och effektiv metod för kvantitativ utvärdering av benmikroarkitektur i en murin osteoporosmodell 

Utvärdering av trabekulär benmikroarkitektur i en osteoporosmusmodell

Our research focused on the effects of traditional Chinese medicine, or TCM, on osteoporosis. We are trying to explore the mechanism, efficacy, and safety of TCM intervention for osteoporosis. Micro-computed tomography imaging has recently become a popular technique for bone morphology assessment.It can provide accurate and comprehensive data on bone structure and density parameters, such as bone volume, fracture thickness, and separation. The skeletal system is the most unique tissue structure in terms of the physical properties of the body. It contains the inorganic component arginine calcium phosphate, and it is hard and stiff as a result.The process of hurting bone symptoms is tedious. We provided a complete and detailed process from modeling to osteoporosis in evaluation for researchers. We believe the application of the technology will undoubtedly bring great improvements to osteoporosis research in the context of ethnomedicine exploration.

Watch this Scientific Journal Video about Analyzing Micro-Computed Tomography CT Images of Murine Trabecular Bone at JoVE.com

Analyzing Micro-Computed Tomography (CT) Images of Murine Trabecular Bone  

Analysera mikrodatortomografi (CT) bilder av murint trabekulärt ben

För att börja analysera mikrodatortomografin, eller mikro-CT-bilderna, i programvaran för mikro-CT-bildsystemet, välj de data som ska analyseras. Klicka på Sub och ställ in pixelstorleken på 10 mikrometer. Flytta och ändra storlek på intresseområdet, eller ROI, så att det inkluderar det distala lårbenet ovanför tillväxtplattan.Klicka sedan på Start. Klicka sedan på Analys 3D knappen för att få den resulterande 3D-rekonstruktionen. Exportera data från mikro-CT-systemet till datorn för analys.När du har importerat rekonstruerade CT-data klickar du på Process- och bildkalkylator. Klicka sedan på Region Pad och Interactive. Justera den gula kryssrutan till lämplig avkastning på investeringen.När du har avslutat bildkalkylatorn väljer du tillägget benmikroarkitekturanalys eller BMA och klickar på Segment Cortex och Segment Trabeculae. Klicka slutligen på Mät ben för att beräkna benmorfometriska index. De trabekulära mikroarkitektoniska parametrarna mättes i möss från två grupper, den skenopererade gruppen och den ovariektomerade osteoporosmodellgruppen, eller OVX-gruppen.Resultaten visade på signifikanta skillnader mellan möss i de två grupperna. Förhållandet mellan benvolym och total vävnadsvolym i OVX-gruppen var 3 % lägre än i skengruppen. Trabekulärtjockleken hos möss i OVX-gruppen var lägre än i skengruppen, och trabekulär separation hos OVX-mössen var större än hos skenmössen.3D-visningar av trabekulära ROI extraherade från rekonstruerad benvolym avslöjade att jämfört med sham-gruppen, i OVX-mössen, var trabeklerna glesa och visade osteoporos.

Research

JoVE Journal

Medicine

Analyzing Micro-Computed Tomography (CT) Images of Murine Trabecular Bone

Trabecular Bone Microarchitecture Evaluation in an Osteoporosis Mouse Model

Bone microstructure refers to the arrangement and quality of bone tissue at the microscopic level. Understanding the bone microstructure of the skeleton ...