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04:02 min
October 27th, 2023
DOI :
10.3791/200638-v
Transcript
首先移出 0.5 毫升寻常小球藻的对数期培养物。将培养物铺在Tris-乙酸盐-磷酸盐或TAP琼脂平板上。在 25 摄氏度下培养培养 5 天。
接下来,在摇瓶中接种 10 毫升补充的 LB 肉汤,其中装满电穿孔根癌农杆菌培养物的环。将烧瓶在 28 至 30 摄氏度和 250 RPM 下孵育过夜。第二天,将 1 毫升过夜培养物接种到 50 毫升补充的 LB 中。将烧瓶在 28 至 30 摄氏度和 250 RPM 下孵育。
共同培养藻类和细菌培养物。首先,将农杆菌培养物转
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