Name: Video: Acute Neurobehavioral Assessments of the Closed-Head Mild Traumatic Brain Injury (MTBI) Rat Model
Uploaded: 2023-09-22T15:00:00
Description: 97 Views. Acute Neurobehavioral Assessments of the Closed-Head Mild Traumatic Brain Injury (mTBI) Rat Model

acute neurobehavioral assessments of the closed-head mild traumatic brain injury (mtbi) rat model

Validating Injury Response and Behavioral Assessments in an mTBI Rat Model

Mild traumatic brain injury (mTBI), or concussion, is the most prevalent type of injury and can lead to various short-term and chronic symptoms1. These symptoms may include dizziness, headaches, depression, and anhedonia, among others, leading to significant suffering for individuals affected by mTBI2. Since most mTBIs are caused by blunt force trauma3, it becomes imperative to develop animal models that accurately mimic such injuries. These models are essential for gaining a better understanding of the injury and its underlying mechanisms, offering a controlled environment with reduced variability and heterogeneity compared to human studies.
Numerous well-established rodent models have been developed for traumatic brain injury (TBI), including fluid percussion injury (FPI)4, controlled cortical impact (CCI)5, weight-drop injury6, blast traumatic brain injury7, and others. However, these models primarily focus on replicating moderate to severe TBI scenarios. In contrast, the experimental models specifically designed to simulate mTBI have received relatively less attention and remain underexplored8. Therefore, there is a critical need to establish a stable and reproducible animal model that accurately represents mTBI. Such a model would significantly enhance our understanding of the neurobiological and behavioral consequences associated with mTBI.
One cannot distinguish the functional deficits in mTBI rats compared to normal rats via casual observation after the effects of anesthesia have worn off. Therefore, it is necessary to administer specific tests. In humans, a wide range of clinical assessments are used to evaluate patients9,10,11. Similarly, establishing a successful model in the rat model also requires using rapid assessment tools to determine its validity.
In this study, we present a closed-head mTBI rat model, enabling the investigation of mTBI in a manner that closely resembles the human condition. The detailed description of the model and its validation procedure provides a comprehensive understanding of the experimental approach utilized in studying mTBI.

Author Spotlight: Advancing Traumatic Brain Injury Research - A Closed-Head Model for Accurate Replication and Rapid Assessment 

Rat Model of Closed-Head Mild Traumatic Injury and its Validation

Watch this Scientific Journal Video about Acute Neurobehavioral Assessments of the Closed-Head Mild Traumatic Brain Injury MTBI Rat Model at JoVE.com

Acute Neurobehavioral Assessments of the Closed-Head Mild Traumatic Brain Injury (MTBI) Rat Model  

Acute Neurobehavioral Assessments of the Closed-Head Mild Traumatic Brain Injury (mTBI) Rat Model

acute-neurobehavioral-assessments-closed-head-mild-traumatic-brain

Research

JoVE Journal

Neuroscience

97 Views. Acute Neurobehavioral Assessments of the Closed-Head Mild Traumatic Brain Injury (mTBI) Rat Model

Video: Acute Neurobehavioral Assessments of the Closed-Head Mild Traumatic Brain Injury (MTBI) Rat Model  

Animal models are crucial for advancing our understanding of mild traumatic brain injury (mTBI) and guiding clinical research. To achieve meaningful insights, developing a stable and reproducible animal model is essential. In this study, we report a detailed description of a closed-head mTBI model and a representative validation method using Sprague-Dawley rats to verify the modeling effect. The model involves dropping a 550 g mass weight from a height of 100 cm directly onto the head of a rat on a destructible surface, followed by a 180-degree turn. To assess the injury, rats underwent a series of neurobehavioral assessments 10 min post-injury, including time of loss of consciousness, first seeking-behavior time, escape ability, and beam balance ability test. During the acute and subacute stages following the injury, behavioral tests were conducted to assess motor coordination ability (Beam task), anxiety (Open Field test), and learning and memory abilities (Morris Water Maze test). The closed-head mTBI model produced a consistent injury response with minimal mortality and replicated real-life situations. The validation method effectively verified the model development and ensured the stability and consistency of the model.

Here, we present a closed-head mild traumatic brain injury (mTBI) rat model and its validation exhibiting remarkable similarity to human mTBI concerning behavioral manifestations during the acute and subacute stages.

Animal models are crucial for advancing our understanding of mild traumatic brain injury (mTBI) and guiding clinical research. To achieve meaningful ...