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JoVE Journal

Bioengineering

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Preparation of Dielectrophoresis Medium and Electrodeformation Prime Medium

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01:06 min

October 13th, 2023

October 13th, 2023

97 Views

Transcript

要制备介电泳或DEP培养基,请使用秤称取12.75克蔗糖和0.45克葡萄糖。将两种粉末溶解在 150 毫升去离子水和 3.5 毫升 PBS 的溶液中。使用低量程电导率测试仪测量电导率以确保其为每米 0.04 西门子。

使用渗透压计,确认渗透压在血浆的正常范围内。将制备的 DEP 培养基储存在 4 摄氏度下。在 15 毫升试管中,将 0.5 克牛血清白蛋白溶解在 10 毫升 DEP 培养基中,并使用涡旋混合器充分混合以制备设备的主要培养基。

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