Preparing a Vascularized Micro-Tumor System Using Human Cells for Studying Tumors and Drug Responses

To begin, place the HBSS cell dissociation reagent EGM-2 and DMEM in a 37 degree Celsius water bath for 10 to 15 minutes. Thaw thrombin and laminin overnight at 4 degrees Celsius and fibrinogen at room temperature. Add 1.5 microliters of thrombin