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DOI :
10.3791/2008-v
•
15:31 min
May 12th, 2010
Chapters
0:00
Title
2:22
Use of High-Quality MISSION esiRNA Libraries for RNAi Screening
3:49
Optimizing Transfection Prior to Screening
1:43
Introduction
5:28
Setting Up the Assay and Preparing for the Primary Screen
9:34
The Primary Screen
12:03
Secondary Screen of the Selected Genes for Hit Validation
13:40
Representative Results Screening for Mammalian Cell Cycle Genes Using MISSION esiRNA
14:43
Conclusion
여기 우리는 세포 분열에 관련된 유전자에 대한 높은 처리량 화면에 인간의 esiRNA 라이브러리를 사용합니다. 우리는 설정하고 esiRNA 화면뿐만 아니라, 어떻게 결과를 분석하고 검증을 실시하는 방법을 보여줍니다.
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