Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
TR - Turkish
JA - Japanese
Please note that all translations are automatically generated. Click here for the English version.
145 Views
•
00:46 min
January 12th, 2024
DOI :
10.3791/200815-v
Transcript
首先,用 3 到 5 毫升 PBS 稀释粘稠的玻璃体液以促进移液。将盒式袋内接收的稀玻璃体液排入 50 毫升管中。对于低体积的房水,将样品转移到 1.5 毫升管中,然后用等体积的 PBS 冲洗原来的 500 微升微量离心管。
将漂洗液加入 1.5 毫升管中。最后,在室温下将玻璃体液以 3000 G 离心 15 分钟。然后小心地将上清液移出到新管中,而不会干扰细胞沉淀。
Sign in or start your free trial to access this content
Explore More Videos
From the series
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Librarians
Access
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved