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Please note that all translations are automatically generated. Click here for the English version.
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02:21 min
January 19th, 2024
DOI :
10.3791/200846-v
Transcript
首先,用 5 微升 Alexa 荧光标记的 GFP 纳米抗体溶液填充每根针头。将 25 x 25 毫米的盖玻片放在载玻片表面上。在载玻片上移取 40 微升卤代烃油,并沿盖玻片的周边铺开。
在注射显微镜下,将针头的尖端放在浸没在油中的盖玻片边缘。相应地调整 PicoPump 的喷射气压。用准备好的果蝇胚胎载玻片替换空载玻片。
将注射针尖垂直于胚胎的前后轴。使用XY阶段操纵器将胚胎引导到针头,直到尖端到达卵黄中心,然后泵送两到三次以将抗体注入卵黄中。使用XY阶段操纵器,
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