JoVE Journal

Developmental Biology

A subscription to JoVE is required to view this content.

Visualizing Low-Abundance Proteins and Post-Translational Modifications in Living Drosophila Embryos via Fluorescent Antibody Injection

This protocol describes the customized antibody-based fluorescence labeling and injection into early Drosophila embryos to enable live imaging of low-abundance proteins or post-translational modifications that are challenging to detect using traditional GFP/mCherry-tag approaches.

Chapters in this video

0:00

Introduction

1:33

Fluorescent Labeling of Antibodies for Imaging Low Abundance Proteins in Drosophila

2:18

Preparation and Alignment of Drosophila Embryos for Injecting Fluorescently Labeled Antibodies

4:56

Injecting Fluorescently Labeled Antibodies in Live Drosophila Embryos and Imaging of Low Abundance Proteins

Related Videos

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More