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Please note that all translations are automatically generated. Click here for the English version.
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02:26 min
October 6th, 2023
DOI :
10.3791/200850-v
* These authors contributed equally
Transcript
首先,取一层长 16 厘米、宽 12 厘米、厚 20 微米的聚乙烯塑料薄膜。使用玻璃研磨工具在标准的 96 孔 PCR 布局中压出 30 个半圆形突起。将压制的聚乙烯塑料薄膜的两面暴露在紫外线下一小时进行消毒。
在半圆形表面上加入少量10%聚乙烯醇。接下来,将大约 3, 000 只麻醉的雌性毛滴虫黄蜂放入收集箱中。将薄膜蛋卡的凸面朝向收集盒,并用橡皮筋固定边缘。
现在向每个突起处加入四微升氨基酸溶液。然后用另一片聚乙烯塑料薄膜覆盖它。用橡皮筋用两张塑料片紧紧地盖住收
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