Ministry of Agriculture

4 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum

Juntao Liu^*1, Lihui Zhu^*1, Lihui Wang^1,2, Yongjun Chen¹, Bikash Ranjan Giri¹, Jianjun Li², Guofeng Cheng¹

¹Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, ²Tianjin Agricultural University

Here, we present a protocol to describe extracellular vesicles (EVs) isolation in in vitro cultured medium from adult Schistosoma japonicum.

Immunology and Infection

Intracranial Subarachnoidal Route of Infection for Investigating Roles of Streptococcus suis Biofilms in Meningitis in a Mouse Infection Model

Shouming Zhang^1,2,3, Xueping Gao^1,2,3, Genhui Xiao^1,2,3,4, Chengping Lu^1,2,3, Huochun Yao^1,2,3, Hongjie Fan^1,2,3,5, Zongfu Wu^1,2,3

¹College of Veterinary Medicine, Nanjing Agricultural University, ²Key Lab of Animal Bacteriology, Ministry of Agriculture, ³OIE Reference Lab for Swine Streptococcosis, ⁴School of Medicine, Tsinghua University, ⁵Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses

Here, we describe the intracranial subarachnoidal route of infection in mice to study roles of biofilms in Streptococcus suis meningitis. This infection model is also suitable for studying the pathogenesis of other bacterial meningitis and the efficacy of new drugs against bacterial meningitis.

Genetics

An Effective Inoculation Method for Phytophthora capsici on Black Pepper Plants

YueFeng Su^*1,2, Rui Fan^*1,3, Li-Song Hu^1,4, Bao-Duo Wu^1,3, Chao-Yun Hao¹

¹Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Science (CATAS), ²Hainan University, ³Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture, ⁴Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops

Pinpricking the basal head of the black pepper plant is a brief and time-saving method to damage it. Here, we provided detailed steps with a video for infecting black pepper plants.

Biochemistry

Extracting Venom from the Parasitoid Wasp Trichogramma dendrolimi Using an Artificial Host

Hai-yan Wang^*1,2, Ze-qi Yu^*1,2, Xin-yue Ren^1,2, Yuan-xi Li^1,2, Zhi-chao Yan^1,2,3

¹Department of Entomology, Nanjing Agricultural University, ²Key Laboratory of Integrated Pest Management of Crop of East China, Ministry of Agriculture, ³Sanya Institute of Nanjing Agricultural University

Here, we present a protocol for extracting venom from Trichogramma dendrolimi using an artificial host created with polyethylene film and amino acid solution.